Collagen-based biomedical material by taking dialdehyde polyethylene glycol as cross-linking agent and preparation method thereof

Abstract

The invention provides a collagen-based biomedical material by taking dialdehyde polyethylene glycol as a cross-linking agent and a preparation method thereof. The preparation method disclosed by the invention comprises the following steps of: preparing dialdehyde polyethylene glycol having a certain hydroformylation degree at first, uniformly mixing dialdehyde polyethylene glycol solution with the mass fraction of 0.1-5% with collagen solution with the mass fraction of 0.3-3%, then, pouring into a mould, freezing at -30-0 DEG C for 1-10 days, taking out, and freeze-drying in a freeze-drying machine for 1-2 days to obtain dialdehyde polyethylene glycol-collagen sponge; and repetitively immersing the sponge in water for 2-4 times, each time for 2-10 h to obtain dialdehyde polyethylene glycol-collagen hydrogel. According to the invention, a freezing polymerizing technology and a freeze drying technology are combined; the mechanical property, the thermal stability and the enzymatic degradation resistant property of collagen are improved by the prepared dialdehyde polyethylene glycol collagen sponge or gel; furthermore, the collagen-based biomedical material disclosed by the invention has the advantages of porosity, hydrophilia, water-retaining property, nontoxicity, good biocompatibility and the like, can be used in the biomedical fields, such as biological scaffolds, cell culture, drug release, burn and wound dressing and the like, and has good market application prospect.

Description

technical field [0001] The invention relates to the field of preparation of biomedical materials, in particular, to a collagen with dialdehyde polyethylene glycol as a crosslinking agent having porosity, hydrophilicity, water absorption and water retention, non-toxicity and good biocompatibility Based biomedical materials and preparation methods thereof. Background technique [0002] In natural protein materials, collagen is a very important animal protein. Due to its good biocompatibility, biodegradability and low immunogenicity, collagen-based materials are widely used in biomedicine and field of biotechnology. However, the mechanical properties, thermal stability, and enzymatic degradation resistance of biomedical materials prepared from collagen alone often cannot meet the requirements of the application; especially the low mechanical properties of collagen usually make it used in the preparation of tissue engineering The stent cannot maintain a relatively fixed shape ...

AI Technical Summary

Problems solved by technology

Chemical cross-linking agents such as glutaraldehyde (CN13187923A) can effectively improve the performance of collagen to meet the requirements of tissue functional scaffold materials, but the Schiff base produced by the reaction of small molecule aldehydes with collagen has poor structural stability, which easily leads to the formation of free small molecule aldehydes. produced, thus limiting the application in the biomedical field

CN101234216A reports that although collagen-chitosan blending modification can improve the mechanical strength and thermal properties of collagen, the degree of improvement is limited due to the interaction of secondary valence bonds such as hydrogen bonds

So far, there are no literature and patent reports about the use of dialdehyde polyethylene glycol and collagen in the preparation of biomedical materials