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Artemisia sacrorum extract as well as preparation method and application thereof

A technology of Artemisia annua and its extract, applied in the field of medicine, can solve the problem of no particularly good solution, and achieve the effect of significant curative effect and wide source.

Active Publication Date: 2015-01-07
朴光春
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

[0005] There is no particularly good way to treat and prevent liver damage in clinical practice

Method used

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  • Artemisia sacrorum extract as well as preparation method and application thereof
  • Artemisia sacrorum extract as well as preparation method and application thereof
  • Artemisia sacrorum extract as well as preparation method and application thereof

Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0027] Example 1. The preparation method of the Artemisia miltiorrhiza extract

[0028] Take 500g of dry Artemisia persicae, soak in water for 0.5-3 hours, heat for 0.5-3 hours, filter to obtain filtrate I, add water to the residue and heat for 0.5-3 hours to obtain filtrate II, combine filtrates I and II to obtain Artemisia persicae water extract . The water extract of Artemisia tennatus is loaded on a pretreated macroporous adsorption resin column, and eluted with water and 10%-95% ethanol in sequence. The 10%-95% ethanol eluate is concentrated and then freeze-dried to dryness to obtain the Artemisia miltiorrhiza extract.

Embodiment 2

[0029] Example 2. Determination of total flavonoids content in the Artemisia wannensis extract

[0030] Flavonoid standard curve:

[0031] Reagents: rutin, methanol

[0032] Preparation of rutin reference solution

[0033] Accurately weigh 5.0mg of rutin, add methanol, dissolve and dilute to 100ml to obtain 50μg / ml.

[0034] Draw rutin reference solution 0, 1.0ml, 2.0ml, 3.0ml, 4.0ml, 5.0ml, 6.0ml, 7.0ml into a 10ml volumetric flask, add methanol to the mark, shake well, and measure the wavelength: 360nm. The result is as follows:

[0035] y=0.1371x+0.0093 R 2 =0.9999

[0036] Preparation of the solution of the test product (Artemisia tennensis 50% ethanol eluate):

[0037]Take 1ml of the sample solution in an evaporating dish, add 1g of polyamide powder for adsorption, evaporate the solvent in a constant temperature drying oven at 50°C, transfer it to a chromatography column, elute with 30ml of petroleum ether, discard the petroleum ether, and then use 100ml The total ...

Embodiment 3

[0042] Example 3. Separation and structure identification of chemical constituents in the Artemisia wannensis extract

[0043] The Artemisia wannensis extract is extracted with dichloromethane, ethyl acetate and other solutions, and the extracts are separated by reversed-phase silica gel column, SephadexLH-20 gel column, preparative HPLC, ODS low-pressure column, etc., and methanol- Gradient elution with water and other systems separated 20 flavonoids. Through spectral analysis and literature comparison, the chemical structures were determined to be: 1) 6-methoxy-quercetin-7-O-β-D-glucoside, 2) quercetin-3-O-β -D glucoside, 3) isorhamnetin, 4) tagetin-3-O-β-D-glucoside, 5) acacetin-7-O-β-D-glucoside, 6) rutin , 7) 5,7,4'-trihydroxyflavone, 8) 3,3',4',5,7-pentahydroxyflavone, 9) 5,7,4'-trihydroxy-3'-methoxy Flavone, 10) kaempferol, 11) 3,5,7,4'-tetrahydroxy-6-methoxyflavone, 12) 3',4',5,7-tetrahydroxyflavone, 13) 5,7, 4'-trihydroxy-6,3'-dimethoxyflavone, 14) 5,7,4'-trihydrox...

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PUM

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Abstract

The invention discloses an artemisia sacrorum extract which contains total-flavone compounds, wherein the total-flavone compounds comprise one or more of 6-methoxy-quercetagetin-7-O-beta-D-glucoside, quercetin-3-O-beta-D-glucoside, isorhamnetin, queretagetin-3-O-beta-D-glucoside, acacetin-7-O-beta-D-glucoside, rutin, 5,7,4'-trihydroxyflavone, 3,3',4',5,7-pentahydroxyflavone, 5,7,4'-trihydroxy-3'-methoxyflavone, kaempferol, 3,5,7,4'-tetrahydroxy-6-methoxyflavone, 3',4',5,7-tetrahydroxyflavone, 5,7,4'-trihydroxy-6,3'-dimethoxyflavone, 5,7,4'-trihydroxy-6-methoxyflavone, quercitrin, 7,3',4'-trimethyl quercetin, 5-hydroxy-7,4'-dimethoxyflavone, 5,4'-dihydroxy-7-methoxyflavone genkwanin, 5,7-dihydroxy-4'-methoxyflavone, 5,7,3'-dihydroxy-4-methoxyflavone and derivatives. The artemisia sacrorum extract has remarkable prevention and treatment effects on liver injury.

Description

technical field [0001] The invention relates to the technical field of medicine, in particular to the extract of Artemisia tennensis and its preparation method and application. Background technique [0002] There are more than 350 species of Artemisia in the family Asteraceae, which are widely distributed in the temperate regions of the northern hemisphere, Europe, Asia, and North America, and a few species are distributed in tropical regions such as Africa, South Asia, and Central America. China (excluding the separated Seriphidium ( Bess.) Poljak.) has more than 170 species, which are produced in various places, among which A. annua L, A. apiacea Hance, A. capillaris Thunb. and A. argyi Levl.etVant. are used as medicine , especially AI is the most widely used, Artemisia annua can cure malaria, and can also make wine cakes. [0003] Artemisia persicae, herb, subshrub or small shrub, often fragrant when kneaded; leaves alternate and often divided; flower heads are small, he...

Claims

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Application Information

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IPC IPC(8): A61K36/28A61P1/16A61P31/14A61P31/20A61P37/02
CPCA61K36/28A61K2236/33A61K2236/331A61K2236/55
Inventor 朴光春元海丹
Owner 朴光春
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