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Ultrasonic-assisted method for degreasing and extracting potentilla anserina polysaccharide

A technology of ultrasonic wave and hemp, applied in the field of medicine and chemical industry, can solve the problems of low yield, long time-consuming and high cost, and achieve the effect of clear pharmacological effect.

Inactive Publication Date: 2014-12-24
段玉虎
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

[0006] The purpose of the present invention is to solve the shortcomings of existing extraction methods such as long time consumption, large amount of solvent, low yield and high cost, and to study the anti-fatigue and fatigue recovery of Juema polysaccharides by using ultrasonic-assisted degreasing to extract Juema polysaccharides role, providing valuable reference for scientific sports

Method used

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  • Ultrasonic-assisted method for degreasing and extracting potentilla anserina polysaccharide
  • Ultrasonic-assisted method for degreasing and extracting potentilla anserina polysaccharide

Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0029] Weigh 200.00g of the dry powder of Potentilla hemp, put it in a 1000ml flask, add 400ml of petroleum ether, reflux for one hour at 70℃, evaporate the solvent and add 400ml of 50% ethanol, soak and wash twice at room temperature, 4h each time, evaporate dry After solvent, add 200ml distilled water, transfer to ultrasonic extraction equipment, 40℃ ultrasonic, power 80W extraction for 20min, filter, collect water extract, filter residue and then add water to ultrasonic extraction twice, combine water extract, rotary evaporation to concentrate to 200ml, add to it Add 2ml of 10μg / ml papain solution for enzymolysis at a temperature of 40°C for 10h, then centrifuge the enzymatic hydrolysate at 1000r / min for 5 minutes, take the supernatant, and filter with a micro filter (pressure 0.05MPa), and further Remove the finer filter residue, concentrate the collected filtrate, and then use a mixture of n-butanol and chloroform with a volume ratio of 1:2, extract 5 times, collect the upp...

Embodiment 2

[0031] Weigh 200.00g dry powder of Potentilla hemp, put it in a 1000ml flask, add 400ml petroleum ether, reflux for one hour at 80℃, evaporate the solvent, add 400ml, 70% ethanol, soak and wash twice at room temperature, 4h each time, evaporate the solvent. Then add 200ml of distilled water, transfer to ultrasonic extraction equipment, 40℃ ultrasonic, 80W power extraction for 20min, filter, collect the water extract, add water to the filter residue and ultrasonically extract twice, combine the water extract, rotary evaporation to concentrate to 200ml, add to it 2ml of 10μg / ml papain solution was enzymatically hydrolyzed at a temperature of 40°C for 10 hours, then the enzymatic hydrolysate was centrifuged at 1000r / min for 5 minutes, the supernatant was taken, and the supernatant was suction filtered (pressure 0.05) to further remove The fine filter residue, the collected filtrate is concentrated, and then extracted with a mixture of n-butanol and chloroform at a volume ratio of 1...

Embodiment 3

[0033] Weigh 200.00g dry powder of Potentilla hemp, put it in a 1000ml flask, add 400ml petroleum ether, reflux for one hour at 70℃, evaporate the solvent, add 400ml, 90% ethanol, soak and wash twice at room temperature, 4h each time, evaporate the solvent. Then add 200ml distilled water, transfer to ultrasonic extraction equipment, 40℃ ultrasonic, power 160W extraction 40min, filter, collect the water extract, add water to the filter residue and ultrasonically extract twice, combine the water extract, rotary evaporation to concentrate to 200ml, add to it 2ml of 10μg / ml papain solution was enzymatically hydrolyzed at a temperature of 40°C for 10 hours, then the enzymatic hydrolysate was centrifuged at 1000r / min for 5 minutes, the supernatant was taken, and the supernatant was suction filtered (pressure is 0.05, and further removed Concentrate the collected filtrate, then use a mixture of n-butanol and chloroform with a volume ratio of 1:3, extract 5 times, collect the upper aque...

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Abstract

The invention provides an ultrasonic-assisted method for degreasing and extracting potentilla anserina polysaccharide. The method comprises: taking potentilla anserine as a raw material, adding petroleum ether, degreasing by employing different temperature for refluxing, after a solvent is volatilized, using ethanol for normal-temperature immersing and washing, adding distilled water, transferring to ultrasonic extraction equipment, performing ultrasonic extraction filtration, collecting the water extraction liquid, adding water into the filter residue and performing ultrasonic extraction for two times, merging the water extraction liquids, performing rotary evaporation condenstation, then adding a papain solution for enzymolysis, centrifuging and getting the supernatant, performing pumping filtration by using a micro pumping filtration instrument to further remove relatively fine filter residue, concentrating the collected filtrate, then using a mixed solution of n-butanol and chloroform to perform extraction for 3-5 times, collecting the upper-layer water phase, adding ethanol into the water phase, standing, centrifuging to collect precipitate, and performing freeze drying to obtain purified potentilla anserina polysaccharide.

Description

Technical field [0001] The present invention relates to the technical field of medicine and chemical industry, in particular to a method for extracting jue ephedra polysaccharides by using ultrasonic assisted degreasing. Background technique [0002] Potentilla is a special economic crop on the Qinghai-Tibet Plateau, which is often used as health care products and Tibetan medicine. Eating potentilla series food can eliminate fatigue, restore physical strength, improve anti-stress ability and anti-anoxia ability, and can regulate human immune function. The medicinal active ingredients of Potentilla angustifolia depend on the polysaccharides, phenolic acids and flavonoids contained in it. Therefore, the extraction of these active ingredients is very important to the medicinal value of Potentilla anserina. [0003] At present, there have been many reports on the extraction method of Potentilla. Potentilla polysaccharides are water-soluble, so the commonly used method is water extrac...

Claims

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Application Information

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IPC IPC(8): C08B37/00A61P39/00A61P39/06
Inventor 段玉虎田晓柱
Owner 段玉虎
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