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Calotropis gigantea seedling tissue culture rapid propagation method with terminal bud as starting material

A technology of horn melon and terminal buds, which is applied in the field of plant tissue culture for seedling production, can solve the problems of abnormal mitogens and increase the frequency of mutant plants, and achieves the effects of avoiding mutant seedlings, reducing the average pollution rate and simple method.

Inactive Publication Date: 2014-12-24
SOUTHWEST FORESTRY UNIVERSITY
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

However, it is easy to produce mutant seedlings through the method of leaf-induced callus redifferentiation and emergence.
This is because the leaves are composed of palisade tissue, spongy tissue, epidermal tissue, etc., and the callus induced by it will have heterogeneity; Cause abnormal mitogens and increase the frequency of mutant plants

Method used

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  • Calotropis gigantea seedling tissue culture rapid propagation method with terminal bud as starting material
  • Calotropis gigantea seedling tissue culture rapid propagation method with terminal bud as starting material
  • Calotropis gigantea seedling tissue culture rapid propagation method with terminal bud as starting material

Examples

Experimental program
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Effect test

Embodiment 1

[0038] (1) Collection of terminal buds: In mid-July 2013, at the ox horn melon base in Yuanjiang County, Yunnan Province, we collected healthy and unlignified branches from an excellent single plant of ox horn melon. The thinned twigs were removed, and the leaves were removed, and brought back to the State Forestry Administration Key Laboratory of Biodiversity Conservation in the Southwest Region of Southwest Forestry University on the same day.

[0039] (2) Terminal bud elongation culture: Rinse the terminal buds with tap water for 5 minutes, then cut them into 4cm-long terminal buds, rinse them once with distilled water, spray alcohol on the surface with 75% alcohol, and then transfer the terminal buds Go to the ultra-clean workbench, disinfect with 0.15% mercury liter for 9 minutes, rinse with sterile water 3 times after disinfection, dry the water with sterile filter paper, remove the petiole, cut off the browned part of the base of the terminal bud and the top, Control the...

Embodiment 2

[0045] Except that in step (3) the average effective reproduction multiple was 5.3 times, three transfers were carried out, and rooting culture was carried out after the number reached 300 bottles, and the rest of the steps were the same as in Example 1.

Embodiment 3

[0047] In embodiment 1, step (5) is: when the root system of tissue culture seedlings to be transplanted and planted is 3 centimeters long, clean the culture medium adhering to the root system, red heart soil: humus: perlite = Mix 2:2:1, use carbendazim aqueous solution to sterilize 3 days in advance, the size of the nutrition bag is 7cm×8cm, use a plastic shed to keep moisture, the humidity is controlled at 80%, the temperature is controlled at 20-30°C, and a small amount is started after 15 days Remove the plastic film and reduce the frequency of watering. The air humidity should be properly reduced to about 70%. After one month, the average survival rate will reach 95%. After meeting the afforestation requirements.

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Abstract

A calotropis gigantea seedling tissue culture rapid propagation method with the terminal bud as a starting material relates to a plant vegetative propagation method. The method includes the cultivation steps of terminal bud elongation, auxiliary bud rapid proliferation, tissue culture rooting transplanting and the like. The auxiliary bud rapid proliferation refers to conducting subculture transplanting on proliferation seedlings and repeating the process to achieve the rapid proliferation. The method is stable in growth coefficient and inheritable character, omits the strong seedling culture stage and the natural light seedling hardening stage after the rooting stage, is simple in operation, low in production cost and capable of meeting the requirement of calotropis gigantea good single plants for industrial production.

Description

technical field [0001] The invention belongs to a method for asexual reproduction of plants, in particular to a plant tissue culture method for seedling production. Background technique [0002] Croissant melon ( Calotropis gigantean ) is a perennial upright shrub belonging to the Asclepiaceae family, with a height of up to 3m. , axillary or terminal. Crow's horn melon is mainly distributed in Yunnan, Sichuan, Guangxi, Guangdong and other provinces in my country. It is resistant to barrenness and drought, and can adapt to the ecologically fragile environments of dry-hot valleys, saline-alkali land, coastal beaches and other tropical and subtropical areas, such as dry-hot valleys in Yunnan. It can grow normally in the barren gravel soil of the area, and blossom and bear fruit. It is an important pioneer tree species in the reconstruction of vegetation in difficult areas. [0003] The fibers in the croissant melon fruit can be processed to obtain fibers with a certain degre...

Claims

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Application Information

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IPC IPC(8): A01H4/00
CPCY02A40/22Y02P60/40
Inventor 唐军荣刘惠民马焕成罗明灿郑元伍建榕王连春
Owner SOUTHWEST FORESTRY UNIVERSITY
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