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Terahertz time-domain spectroscopy-based unmarked hemagglutinin detection method

A technology of hemagglutinin protein and terahertz time domain, which is applied in measurement devices, biological tests, material inspection products, etc., can solve the problems of long detection time and low detection efficiency, and achieves improved detection sensitivity, reduction of operation steps, and savings The effect of detection time

Active Publication Date: 2014-12-17
SHENZHEN UNIV
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Problems solved by technology

[0004] In view of the above-mentioned deficiencies in the prior art, the purpose of the present invention is to provide a detection method for unlabeled hemagglutinin protein based on terahertz time-domain spectroscopy, which aims to solve the problems of long detection time and low detection efficiency of the existing detection methods

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  • Terahertz time-domain spectroscopy-based unmarked hemagglutinin detection method
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  • Terahertz time-domain spectroscopy-based unmarked hemagglutinin detection method

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Embodiment Construction

[0021] The present invention provides a non-labeled hemagglutinin protein detection method based on terahertz time-domain spectroscopy. In order to make the purpose, technical solution and effect of the present invention clearer and clearer, the present invention will be further described in detail below. It should be understood that the specific embodiments described here are only used to explain the present invention, not to limit the present invention.

[0022] The unlabeled hemagglutinin protein detection method based on terahertz time-domain spectroscopy of the present invention comprises steps:

[0023] a. The sample is dissolved in PBS buffer solution to prepare different concentrations of antigen solution H9, and the monoclonal antibody F10 is reacted with the antigen solution H9 in an equal volume ratio to obtain the reactant;

[0024] b. Incubate the reactants at room temperature for a period of time, then shake, and overnight at low temperature to shape the structur...

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Abstract

The invention discloses a terahertz time-domain spectroscopy-based unmarked hemagglutinin detection method which comprises the following steps: a. dissolving a sample into a PBS buffer solution to prepare an antigen solution H9 with different concentration, and reacting a monoclonal antibody F10 with the antigen solution H9 according to an equal volume ratio to obtain a reactant; b. incubating the reactant at room temperature for a certain period of time, then shaking, and staying overnight under a low-temperature condition, thereby forming an antigen-antibody complex structure; c. placing the complex into a detection box, vertically placing the detection box at the focal spot of a terahertz light beam, and recording concentration-related terahertz intensity absorption spectrum; and d. according to the concentration-related terahertz intensity absorption spectrum, judging whether an absorption peak exists, and if not, detecting hemagglutinin in the sample.

Description

technical field [0001] The invention relates to the technical field of detection, in particular to a method for detecting unlabeled hemagglutinin protein based on terahertz time-domain spectroscopy. Background technique [0002] Influenza virus is an RNA virus that can cause acute upper respiratory tract infection in mammals and poultry. At present, the detection, prevention and control of the virus and its various subtypes has become a medical problem facing the world. There are two main types of influenza virus surface antigens: hemagglutinin protein and sialidase. Among them, the distribution of hemagglutinin protein accounts for 80% of the surface of the virus, so the detection of hemagglutinin protein can improve the sensitivity of influenza virus recognition. Most of the detection of hemagglutinin protein in the prior art adopts biochemical immunological methods, such as fluorescent immunoluminescence, western blotting, and enzyme-linked immunosorbent assay. While ...

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Application Information

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Patent Type & Authority Applications(China)
IPC IPC(8): G01N33/68G01N33/577
CPCG01N33/577G01N33/68
Inventor 孙怡雯钟俊兰
Owner SHENZHEN UNIV
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