Kit for detecting foot-and-mouth disease type-A virus as well as preparation and using methods of kit

Abstract

The invention discloses a kit for detecting a foot-and-mouth disease type-A virus, a preparation method of the kit and a using method of the kit used for achieving aims except for disease diagnosis. Primers for amplifying the foot-and-mouth disease type-A virus have four gene sequences; and the kit for detecting the foot-and-mouth disease type-A virus internally comprises the four primers with the gene sequences. Relevant experiments prove that nucleic acid capable of specifically and rapidly amplifying the foot-and-mouth disease type-A virus is capable of detecting a specific band through nucleic acid electrophoresis and also has specificity. The relevant experiments hint that the sequences can be applied to preparation of GeXP diagnosis kits for rapidly identifying the foot-and-mouth disease type-A virus and epidemiologic study of the foot-and-mouth disease virus.

Description

technical field [0001] The invention relates to a kit for detecting foot-and-mouth disease type A virus, a preparation method of the kit and a method for using the kit for non-disease diagnosis purposes. Background technique [0002] Foot-and-mouth disease (FMD) is an acute and severe infectious disease of animals caused by foot-and-mouth disease virus (FMDV), which mainly harms pigs, cattle, and sheeppox cloven-hoofed animals, with a very high incidence , has caused huge political and economic losses, so it is listed as the first class A infectious disease by the World Health Organization (OIE). FMDV can be divided into 7 serotypes, namely A, O, C, Asia 1, SAT 1, SAT 2 and SAT 3, and each serotype has many different subtypes, and each serotype There is no cross-immunity between them, and there is only partial cross-immunity between subtypes of the same serotype. [0003] Foot-and-mouth disease (FMD) is an acute and severe infectious disease of animals caused by foot-and-m...

AI Technical Summary

Problems solved by technology

The sensitivity of the test strip to detect the FMDV antigen in the epithelial suspension test is the same as that of the conventional antigen ELISA, and has 100% equivalent sensitivity to the FMDV serotypes O, A, C and Asia 1 in the cell culture supernatant sex, but cannot distinguish between these serotypes

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