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Detection kit for measuring content of urea without interference of endogenous ammonia in serum

A technology for detecting kits and urea content, applied in the measurement of color/spectral characteristics, etc., can solve problems such as interference and poor stability of reagents, and achieve the effects of high accuracy, good precision and improved stability

Inactive Publication Date: 2014-12-10
上海睿康生物科技有限公司
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

However, this method is seriously interfered by the endogenous ammonia in the sample, and the stability of the reagent is poor.

Method used

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  • Detection kit for measuring content of urea without interference of endogenous ammonia in serum
  • Detection kit for measuring content of urea without interference of endogenous ammonia in serum
  • Detection kit for measuring content of urea without interference of endogenous ammonia in serum

Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0035] The composition of the assay kit is as follows:

[0036] 1. Reagent R1 is:

[0037]

[0038] 2. Reagent R2 is:

[0039]

[0040] Components can be added sequentially at room temperature, added simultaneously, or individually packaged and reconstituted immediately prior to testing.

[0041] 3. The reagent detection ratio is 4:1

[0042]The CRE detection kit described in this example is applicable to various types of automatic biochemical analyzers, taking Hitachi 7170 automatic biochemical analyzer as an example, its operation is shown in Table 1. Analysis method: Two-point endpoint method, that is, the amount of reagents R1 and R2 are 240ul and 60ul respectively, and the sample volume is 3.5ul; add 3.5ul sample to 240ul reagent R1 and incubate at 37°C for 5min, add 60ul R2, incubate at 37°C for 1.5min, continuous Monitor the change of absorbance for 180s, and calculate △A / min; the main detection wavelength is 340nm, and the secondary wavelength is 405nm.

[00...

Embodiment 2

[0047] The composition of the assay kit is as follows:

[0048] 1. Reagent R1 is:

[0049]

[0050] 2. Reagent R2 is:

[0051]

[0052] Components can be added sequentially at room temperature, added simultaneously, or individually packaged and reconstituted immediately prior to testing.

[0053] 3. The reagent detection ratio is 4:1

[0054] The CRE detection kit described in this example is applicable to various types of automatic biochemical analyzers, taking Hitachi 7170 automatic biochemical analyzer as an example, its operation is shown in Table 1. Analysis method: Two-point endpoint method, that is, the amount of reagents R1 and R2 are 240ul and 60ul respectively, and the sample volume is 3.5ul; add 3.5ul sample to 240ul reagent R1 and incubate at 37°C for 5min, add 60ul R2, incubate at 37°C for 1.5min, continuous Monitor the change of absorbance for 180s, and calculate △A / min; the main detection wavelength is 340nm, and the secondary wavelength is 405nm.

Embodiment 3

[0056] The composition of the assay kit is as follows:

[0057] 1. Reagent R1 is:

[0058]

[0059] 2. Reagent R2 is:

[0060]

[0061]

[0062] Components can be added sequentially at room temperature, added simultaneously, or individually packaged and reconstituted immediately prior to testing.

[0063] 3. The reagent detection ratio is 4:1

[0064] The CRE detection kit described in this example is applicable to various types of automatic biochemical analyzers, taking Hitachi 7170 automatic biochemical analyzer as an example, its operation is shown in Table 1. Analysis method: Two-point endpoint method, that is, the amount of reagents R1 and R2 are 240ul and 60ul respectively, and the sample volume is 3.5ul; add 3.5ul sample to 240ul reagent R1 and incubate at 37°C for 5min, add 60ul R2, incubate at 37°C for 1.5min, continuous Monitor the change of absorbance for 180s, and calculate △A / min; the main detection wavelength is 340nm, and the secondary wavelength is ...

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Abstract

The invention discloses a detection kit for measuring the content of urea without the interference of endogenous ammonia in serum. The detection kit comprises a reagent R1 and a reagent R2, wherein the reagent R1 contains 1-500mmol / L buffer solution with the pH value of 3.0-8.0, 1-80KU / L glutamate dehydrogenase, 0.5-2KU / L NADH, 1-100mmol / L alpha-oxoglutarate, 0.1-0.5% of a surface active agent and 0.1-1g / L of preservative; the reagent R2 contains 1-500mmol / L buffer solution with the pH value of 3.0-8.0, 1-60KU / L urease, 0.5-2KU / L NADH, 1-100mmol / L alpha-oxoglutarate, 10-500mmol / L sodium chloride, 1-50mmol / L enzyme protective agent, and 0.1-1g / L preservative. The kit can eliminate the interference of the endogenous ammonia and has the characteristics of excellent stability and strong anti-interference ability.

Description

technical field [0001] The invention specifically relates to a detection kit for measuring urea content which is not interfered by serum endogenous ammonia. Background technique [0002] Clinically, urea (Urea) is used to diagnose glomerulonephritis, advanced kidney disease, renal failure, chronic pyelonephritis, prostate enlargement, urinary tract stones, urethral stricture, and bladder tumor causing urethral compression, etc. [0003] Determination methods of urea include: chemical method, urease-Bo's method, etc. The chemical method can be further divided into: (1) diacetyl-oxime color method, the principle is: urea can react with diacetyl, and under the condition of strong acid heating, a pink diazine compound is generated, which is colorimetric at 540nm. Unstable, commonly used instead of diacetyl-oxime, which is hydrolyzed into diacetyl in case of acid. This method has poor specificity, narrow linear range, and the reagents contain strong chemicals, which are easy to...

Claims

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Application Information

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IPC IPC(8): G01N21/33
Inventor 李伟奇陈瑛房君江张秀文林清玉
Owner 上海睿康生物科技有限公司
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