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Specific primer for amplifying COI genes of mitochondria of lepidoptera insects

A lepidopteran insect and mitochondrial technology, applied in the direction of DNA preparation, DNA / RNA fragments, recombinant DNA technology, etc., to achieve the effects of shortening the experimental cycle, good sequencing peaks, and reducing experimental costs

Active Publication Date: 2014-11-26
YANGZHOU UNIV
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Problems solved by technology

At present, there is no relevant report on the specific primers for amplifying the COI gene of Lepidoptera insects in the world

Method used

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  • Specific primer for amplifying COI genes of mitochondria of lepidoptera insects
  • Specific primer for amplifying COI genes of mitochondria of lepidoptera insects
  • Specific primer for amplifying COI genes of mitochondria of lepidoptera insects

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Embodiment Construction

[0016] The amplification primer screening method of the Lepidoptera COI gene of the present invention is divided into two steps: 1. Based on the sequence of the COI gene in the mitochondrial genome sequence of the Lepidoptera insect that has been measured, find out the COI gene by comparison Design primers for relatively conserved sequence fragments; 2. Amplify the COI gene of Lepidoptera insects with the synthesized primers, and test their versatility and amplification efficiency in Lepidoptera.

[0017] Below in conjunction with example the invention is further described:

[0018] 1. Sample collection: In August 2013, we collected insects from different families of Lepidoptera, mainly involving important pests in agriculture and forestry, including Noctuidae, Mothidae and Luphidae.

[0019] 2. DNA extraction: Axygen kit to extract DNA, refer to the instructions for specific steps.

[0020] 3. Data source: The data comes from the National Center for Biotechnology Information...

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Abstract

The invention relates to a primer pair for amplifying COI (cytochrome c oxidase I) genes of mitochondria of lepidoptera insects, wherein the sequence of the primer pair is shown in SEQ ID NO. 1 and SEQ ID NO. 2. By using the amplification primer disclosed by the invention, the COI genes of various lepidoptera species can be effectively amplified, a large-scale genetic background analysis is carried out on significant agriculture and forest insects in lepidoptera, some affinis species which are difficult to authenticate are accurately authenticated, and an important tool is provided for species authentication, phylogeny, population genetic structure, community evolution history and geographical population authentication for lepidoptera insects in China.

Description

technical field [0001] The invention relates to a pair of specific primers for amplifying mitochondrial cytochrome c oxidase I (cytochrome c oxidase I; COI) gene of lepidoptera insects. Background technique [0002] Lepidoptera has more than 40 superfamilies, more than 120 families and 180,000 species. It is the second largest order after Coleoptera, including butterflies and moths. Lepidoptera insects belong to complete metamorphosis. The development process is divided into four stages: egg, larva, pupa and adult. It is an important model organism for the study of insect mimicry, relationship with host plants, wing pattern development biology and insect physiology. [0003] The vast majority of Lepidoptera insects are agricultural and forestry pests. Smaller ones often roll leaves, attach leaves, weave silk and form nets, or drill into plant tissues to feed on them. Larger ones often eat up leaves, bite off roots, or drill branches. . On the other hand, many butterfly and...

Claims

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Application Information

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Patent Type & Authority Applications(China)
IPC IPC(8): C12N15/11C12N15/10
Inventor 陆明星汤小天杜予州
Owner YANGZHOU UNIV
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