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A cotton fiber-specific mybl1 gene

A gene and gene coding technology, applied in genetic engineering, plant genetic improvement, angiosperm/flowering plants, etc., can solve the problems of poor cotton fiber quality and inability to use high value-added and high-quality textile products to produce raw materials, etc.

Inactive Publication Date: 2016-07-13
HUAZHONG NORMAL UNIV
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  • Abstract
  • Description
  • Claims
  • Application Information

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Problems solved by technology

my country's cotton output ranks first in the world, but the quality of cotton fiber is poor and cannot be used as raw materials for some high-value-added high-quality textile products

Method used

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  • A cotton fiber-specific mybl1 gene
  • A cotton fiber-specific mybl1 gene
  • A cotton fiber-specific mybl1 gene

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Embodiment Construction

[0040] 1. Genomic DNA sequence and cDNA isolation and cloning of cotton GhMYBL1 gene and promoter

[0041] A full-length cDNA sequence of a new MYB family gene is isolated from the cotton fiber cDNA library (by LiXB et al., 2002, Molecular characterization of the cottonGhTUB1 gene that is preferentially expressed in fiber.Plant Physiol 130: 666-674), as shown in SEQ.ID.No.2, named for GhMYBL1. The amino acid sequence of the encoded protein is shown in SEQ.ID.No.3. In order to obtain the genomic DNA sequence of the GhMYBL1 gene, design and synthesize primers at both ends of the open reading frame (ORF) according to the above cDNA sequence, and use cotton genomic DNA as a template, at 94 ° C for 5 min, 1 cycle; at 94 ° C for 1 min, 58 1 min at °C, 2 min at 72 °C, 32 cycles; finally, a 10 min extension at 72 °C was amplified to obtain a DNA fragment of about 1.5 kb (the sequence of the gene coding region, excluding the promoter sequence). This DNA fragment was inserted into pBS...

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Abstract

The present invention relates to a cotton fiber specific MYBL1 gene. The full length of the gene sequence is 3789bp, see SEQ ID No.1 sequence, wherein, the 2017bp fragment before the initiation codon (ATG) is the promoter sequence, and there are two intron insertions in the gene coding region, which are respectively inserted at the 45th and 88 codon sites. The cDNA of the gene is 1264 bp in length, see SEQ ID No.2 sequence, wherein the open reading frame is 975 bp, and encodes a protein containing 324 amino acids. GhMYBL1 Specifically expressed during the secondary wall synthesis of cotton fiber cells, GhMYBL1 protein can form homodimers. Overexpressed in Arabidopsis GhMYBL1 lead to thickening of the cell walls of interbundle fiber cells, vessel cells and lignified fiber cells in the xylem of mature stems of transgenic Arabidopsis thaliana, indicating that GhMYBL1 To a certain extent, it affects the synthesis of plant cell secondary wall components. The above results show that GhMYBL1 It may be involved in the regulation of the secondary wall development of cotton fiber cells. Therefore, the gene and its fiber-specific promoter may have important application value for improving cotton fiber quality.

Description

Technical field: [0001] The invention relates to a cotton gene and its specific regulatory element (promoter), in particular to a new member gene MYBL1 in the cotton R2R3MYB family, which is further applied to the improvement of cotton fiber quality. Background technique: [0002] The cell wall is the membrane covering the protoplast at the outermost layer of the cell. As a complex dynamic structure, the plant cell wall plays an important role in the development of plants. Secondary cell wall (SCW) exists in plant cells that undergo secondary growth. The secondary cell wall is a layer of thickened structure that continues to accumulate inside the primary cell wall (PCW) after plant cells stop growing. The main component is cellulose. , hemicellulose and lignin. Secondary cell walls store a large amount of solar energy and carbon sources fixed by plant photosynthesis, and play an important role in human life, providing important raw materials such as edible fiber, textile fi...

Claims

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Application Information

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Patent Type & Authority Patents(China)
IPC IPC(8): C12N15/29C12N15/82C07K14/415A01H5/00
Inventor 李学宝龚思颖孙翔聂晓莹
Owner HUAZHONG NORMAL UNIV
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