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A large-scale preparation method of fish scale type I collagen peptide

A collagen peptide and collagen technology, which is applied in the field of large-scale preparation of fish scale type I collagen peptide (also known as oligopeptide), can solve the problems of complex extracted product components, troublesome purification and poor uniformity, etc. High yield, simple and feasible method, and short production cycle

Active Publication Date: 2018-10-09
THIRD INST OF OCEANOGRAPHY STATE OCEANIC ADMINISTATION
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

Using acid and alkali hydrolysis to extract collagen peptides, the molecular weight distribution of the product is wide and the uniformity is poor. In order to prepare collagen peptides with small molecular weights, a large amount of acid and alkali must be consumed in the extraction process, and the extracted product components are very complicated. Follow-up Purification is troublesome

Method used

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Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0033] A preparation method of fish scale type I collagen peptide, the steps are as follows:

[0034] (1) Pretreatment

[0035] Put marine fish scales or freshwater fish scales into the reaction kettle, add alkaline solution 10 times the weight of fish scales, stir for 1 hour, wash with water, add acid solution 10 times the weight of fish scales, stir for 2 hours, and then use deionized water Wash, wherein the mass volume concentration of alkali solution is 3%, and the mass volume concentration of acid solution is 5%;

[0036] (2) Enzymatic extraction

[0037] Put the pretreated and washed fish scales in a reaction kettle, add deionized water, add concentrated lye to adjust the pH to 6.5, add papain with 2% weight of fish scales, and react for 3 hours at a reaction temperature of 55°C; After the hydrolysis, the temperature was raised to 70° C., and the enzyme was inactivated by keeping the temperature for 20 minutes to obtain the enzymatic hydrolysis solution of fish scale t...

Embodiment 2

[0043] A preparation method of fish scale type I collagen peptide, the steps are as follows:

[0044] (1) Pretreatment

[0045] Crush the fish scales of marine fish or freshwater fish first, then put them into the reaction kettle, add an alkali solution 10 times the weight of the fish scales, stir for 3 hours, wash with water, add an acid solution 10 times the weight of the fish scales, stir for 6 hours, and then Wash with deionized water, wherein the mass volume concentration of the alkali solution is 1%, and the mass volume concentration of the acid solution is 4%;

[0046] (2) Enzymatic extraction

[0047] Put the pretreated and washed fish scales in a reaction kettle, add deionized water, add concentrated lye to adjust the pH to 7.0, add 3% neutral protease by weight of fish scales, and react at a temperature of 50°C for 4 hours; After the enzymatic hydrolysis, the temperature was raised to 75°C, and the enzyme was incubated for 20 minutes to inactivate the enzyme to obt...

Embodiment 3

[0053] A preparation method of fish scale type I collagen peptide, the steps are as follows:

[0054] (1) Pretreatment

[0055] Put marine fish scales or freshwater fish scales into the reaction kettle, add an alkaline solution 10 times the weight of fish scales, stir for 12 hours, wash with water, add acid solution 10 times the weight of fish scales, stir for 12 hours, and then use deionized water Wash, wherein the mass volume concentration of alkali solution is 4%, and the mass volume concentration of acid solution is 6%;

[0056] (2) Enzymatic extraction

[0057] Put the pretreated and washed fish scales in a reaction kettle, add deionized water, add concentrated lye to adjust the pH to 6.5, add flavor protease with 2.5% of the weight of the fish scales, and react at a temperature of 53°C for 3 hours; After the hydrolysis, the temperature was raised to 75° C., and the enzyme was inactivated by keeping it warm for 20 minutes to obtain the enzymatic hydrolysis solution of f...

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Abstract

The invention relates to a large-scale preparation method of fish scale type I collagen peptides. The preparation method comprises the following steps: firstly, performing alkali and acid pretreatment on marine fish scales or freshwater fish scales serving as raw materials to remove impurities, and directly performing directed enzymatic hydrolysis on collagen type I in the fish scales by adopting a one-step biological enzymatic hydrolysis technology to form collagen peptides type I with relatively centralized molecular weight distribution; secondly, separating, purifying and concentrating directed enzyme liquid at the normal temperature by adopting a continuous centrifugal separation and membrane separation combined technology to obtain a fish scale type I collagen peptide solution with purity of not less than 95% and molecular weight of less than 1,000 Dalton; finally, quickly drying finished fish scale type I collagen peptides by adopting a spray-drying technology. The method is simple and feasible in overall process, efficient, energy-saving, short in production cycle and suitable for large-scale production; a fish scale type I collagen peptide product produced by adopting the method is high in purity and good in quality, and the content of peptides with molecular weight of less than 1,000 Dalton is more than 97%.

Description

technical field [0001] The invention relates to a large-scale preparation method of fish scale type I collagen peptide (also called oligopeptide) with a molecular weight below 1000 Daltons, belonging to the technical field of biological extraction. Background technique [0002] In the past, the raw materials for the preparation and production of collagen peptides mainly came from the bones and skins of terrestrial animals. In recent years, due to the occurrence and prevalence of infectious diseases such as mad cow disease and foot-and-mouth disease, people have doubted the safety of collagen peptides derived from livestock. In addition, in areas where Islam and Islam are believed, collagen peptides derived from livestock Due to religious reasons, the application of peptides will also be greatly restricted. In the process of aquatic product processing in such a large fishery country as my country, a large amount of waste - fish scales will inevitably be produced, causing envir...

Claims

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Application Information

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Patent Type & Authority Patents(China)
IPC IPC(8): C12P21/06C07K1/34
CPCY02P20/10
Inventor 陈思谨易瑞灶陈晖郭洪辉谢全灵陈俊德孙继鹏郑美华高然许诺华
Owner THIRD INST OF OCEANOGRAPHY STATE OCEANIC ADMINISTATION
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