A method for measuring foot-and-mouth disease synthetic peptide vaccine emulsion particles

Abstract

The invention relates to a method for measuring foot-and-mouth disease synthetic peptide vaccine emulsion particles. Using white oil as a dispersant, the vaccine emulsion particles and white oil are mixed at a volume ratio of 1:8-1:12, and the vaccine emulsion particles are dispersed and diluted in white oil. The oil is used as the sample to be tested; the detection unit of the laser particle size analyzer is preheated at room temperature for 30-60min; The sample injection pool is placed in the measurement window of the detection unit for particle size analysis and measurement. Compared with the prior art, the invention provides quality standards and inspection methods for measuring the uniformity and stability of the vaccine, objectively judges the uniformity and stability of the vaccine, and eliminates various subjective factors.

Description

technical field [0001] The invention relates to a method for measuring vaccine emulsion particles, in particular to a method for measuring foot-and-mouth disease synthetic peptide vaccine emulsion particles. Background technique [0002] Foot-and-mouth disease synthetic peptide antigen and adjuvant are prepared in a certain proportion and emulsified to make a foot-and-mouth disease synthetic peptide vaccine. The size of the emulsion particles formed by the antigen and adjuvant during the emulsification process directly affects the uniformity and stability of the foot-and-mouth disease synthetic peptide vaccine. The uniformity and stability of the vaccine will directly affect the effect and stability of the vaccine. At present, there are no quality standards and inspection methods for the uniformity and stability of the vaccine in the biological product regulations of the China Veterinary Feed and Drug Administration. The traditional inspection method in the industry is Visua...

AI Technical Summary

Problems solved by technology

[0002] Foot-and-mouth disease synthetic peptide antigen and adjuvant are prepared in a certain ratio and then emulsified to make a foot-and-mouth disease synthetic peptide vaccine. The size of the emulsion particles formed by the antigen and adjuvant during the emulsification process directly affects the uniformity and stability of the foot-and-mouth disease synthetic peptide vaccine. The uniformity and stability of the vaccine directly affect the effect and stability of the vaccine. At present, there are no quality standards and inspection methods for the uniformity and stability of the vaccine in the biological product regulations of the China Veterinary Feed and Drug Administration. The traditional inspection method in the industry is Visual inspection is to judge the uniformity and stability of the vaccine by observing the appearance of the vaccine (color and the layering of the vaccine after standing for a period of time). This traditional visual inspection method is subjective, not scientific enough, and often Causes the uncertainty of the test results, let alone reflects the real situation of the vaccine emulsion particles microscopically