A kind of mango cut leaf weevil attractant and preparation method thereof
A mango leaf-cutting weevil and attractant technology, which is applied in the direction of attracting pests, botanical equipment and methods, biocides, etc., to achieve the effects of convenient transportation, improved trapping efficiency, and low dosage
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Embodiment 1
[0031] One, the preparation of mango cut leaf weevil attractant formula
[0032] 1. Preparation of 1100 μg / ml single component solution
[0033] Preparation of 100 μg / ml valencene solution: Add 10 μl of the previously prepared 10 μg / μl valencene quasi-compound into 990 μl of liquid paraffin, and mix evenly;
[0034] Preparation of 100 μg / ml β-myrcene solution: Add 10 μl of the previously prepared 10 μg / μl β-myrcene standard compound into 990 μl of liquid paraffin, and mix evenly;
[0035] Preparation of 100 μg / ml terpineolene solution: Add 10 μl of the previously prepared 10 μg / μl terpineolene standard compound into 990 μl of liquid paraffin, and mix evenly;
[0036] Preparation of 100 μg / ml β-caryophyllene solution: Add 10 μl of the previously prepared 10 μg / μl β-caryophyllene standard compound into 990 μl of liquid paraffin, and mix evenly;
[0037] Preparation of 100 μg / ml of 100 μg / ml of citrusene solution: 10 μl of the 10 μg / μl of citrusene standard compound prepared ...
Embodiment 2
[0048] The Y-type olfactory instrument was used to measure the directional behavior response of the mango cut leaf weevil to the mixture (improved by our laboratory based on the basic principle of the olfactory instrument, the authorized patent number is: ZL201020239767.7). The main arm of the Y-shaped tube is 20cm, the length of the two arms is 10cm, and the angle between the two arms is 90°C. At the ends of the tubes of the two arms, silicone tubes were used to connect the distilled water bottle and the activated carbon bottle in sequence. Finally, the activated carbon bottle was opened in the atmosphere, and the insect release bottle was connected to the suction device with gauze. Cut the filter paper strip into 5cm×1cm, fold it into a "V" shape, and place it in the trap inlet glass tube. Take 100 μl of the mixture obtained in the above step 1.2 and drop evenly on the filter paper strip and put it into the flavor source bottle on one side, and use the filter paper dripped w...
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