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Moschus anhuiensis microsatellite loci, primers and application of primers

A microsatellite locus and satellite technology, applied in Anhui musk deer microsatellite loci, primers and their application fields, can solve the problems of difficulty in sample collection, difficult to find, and the scarcity of Anhui musk deer populations.

Inactive Publication Date: 2014-10-08
ANHUI UNIVERSITY
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Problems solved by technology

[0003] At present, the Anhui musk deer population is scarce, and it is difficult to find it in field surveys, which makes it very difficult to collect samples. At present, the most suitable non-destructive sampling method is to collect feces

Method used

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  • Moschus anhuiensis microsatellite loci, primers and application of primers
  • Moschus anhuiensis microsatellite loci, primers and application of primers
  • Moschus anhuiensis microsatellite loci, primers and application of primers

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Embodiment Construction

[0012] The present invention will be described in detail below in conjunction with specific embodiments.

[0013] 1. DNA extraction for library construction

[0014] Since high-quality templates are required for library construction and screening of microsatellite sites, we clipped Anhui musk deer muscle tissue (from poached samples collected by the Forestry Bureau) and extracted DNA as templates for the microsatellite enrichment library. Genomic DNA was extracted using SDS / proteinase K cleavage and phenol-chloroform extraction (Sambrook et al., 1999).

[0015] 2. Construction of Anhui Musk Deer Microsatellite Enrichment Library

[0016] Genomic DNA was extracted, and AFLP rapid isolation method (Fast Isolation by AFLP of Sequences Containing repeats, FIASCO) was used to construct a microsatellite enrichment library. The specific process was as follows: about 250 ng of genomic DNA was taken, digested with MseI, and simultaneously treated with AFLP of MseI. Body linker (5'-TA...

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Abstract

The invention discloses Moschus anhuiensis microsatellite loci, primers and application of primers, Moschus anhuiensis microsatellite loci comprise nine stable polymorphic microsatellite loci of which the sequences are respectively shown in SEQ ID NO: 1-SEQ ID NO: 9. The primers of Moschus anhuiensis microsatellite loci have sequences shown in SEQ ID NO: 10-SEQ ID NO: 27. By virtue of the primers, seven individual genotyping data show that the number of alleles is 0-4, the expected heterozygosity He is 0-0.779 and the observed heterozygosity Ho is 0-0.714. Except for the locus MaI75, other loci exhibit polymorphism and meet the requirement of the individual identification.

Description

technical field [0001] The invention relates to biotechnology, in particular to Anhui musk deer microsatellite sites, primers and applications thereof. Background technique [0002] Anhui musk deer belongs to Artiodactyla, Ruminantia, Pecora, and Moschidae, and is a unique mammal in the Palearctic. Only distributed in the Dabie Mountains at the junction of Anhui, Henan, and Hubei provinces, it is a precious wild animal resource in East China. Currently, the total number of populations is around 500-600. Molecular biological information provides a certain theoretical basis for the establishment of conservation units for modified species. However, the research in this area is still blank. Therefore, there is no need to delay the genetic research of Anhui musk deer. [0003] At present, the Anhui musk deer population is scarce, and it is difficult to find it in field surveys, which makes it very difficult to collect samples. At present, the most suitable non-destructive sam...

Claims

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Application Information

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Patent Type & Authority Applications(China)
IPC IPC(8): C12N15/11C12Q1/68
Inventor 张保卫丁玲王慧潘涛
Owner ANHUI UNIVERSITY
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