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Brucella omp31 antigenic epitope and monoclonal antibody thereof, and application of brucella omp31 antigenic epitope and monoclonal antibody thereof

A monoclonal antibody and Brucella technology, applied in the direction of bacterial antigen components, antibodies, antibody medical components, etc., can solve the problem of inability to identify diagnostic methods, affect brucellosis detection and vaccine immunity prevention, and affect attenuated vaccines Strain immune effect and other issues, to achieve the effect of good specific recognition ability

Inactive Publication Date: 2014-10-08
SOUTHERN MEDICAL UNIVERSITY
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  • Abstract
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Problems solved by technology

If the omp31 gene carried by the M5-90 vaccine strain is completely knocked out, the immune effect of the attenuated vaccine strain will be affected
In addition, because the existing diagnostic methods cannot distinguish between animals immunized by M5-90 and animals naturally infected by wild virus strains, it has seriously affected the detection of brucellosis and the prevention of vaccine immunity.

Method used

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  • Brucella omp31 antigenic epitope and monoclonal antibody thereof, and application of brucella omp31 antigenic epitope and monoclonal antibody thereof
  • Brucella omp31 antigenic epitope and monoclonal antibody thereof, and application of brucella omp31 antigenic epitope and monoclonal antibody thereof
  • Brucella omp31 antigenic epitope and monoclonal antibody thereof, and application of brucella omp31 antigenic epitope and monoclonal antibody thereof

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Embodiment Construction

[0028] Below in conjunction with embodiment, further illustrate the present invention.

[0029] The Brucella M5-90 vaccine strain used in the present invention is purchased from Lanzhou Veterinary Research Institute and is a well-known vaccine strain in the industry.

[0030] Construction of omp31 recombinant protein expression vector

[0031] Using the Brucella genome provided by Professor Chen Chuangfu of Shihezi University in Xinjiang as a template to amplify the target gene sequence, use the Brucella ovis omp31 gene sequence provided in GenBank, GenBank accession No.gi|333601402, and use the biological software Oligo7 to design primers for amplification The omp31 gene with 19 amino acid signal peptides truncated at the N-terminus was selected as a PET-30a vector, and Nde I and Xho I restriction sites were introduced at the 5' end and 3' end respectively.

[0032] Upstream primer F: 5-GGAATTC CATATG GCCGACGTGGTTG-3 (SEQ ID NO: 28)

[0033] Downstream primer R: 5-CCG...

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Abstract

The invention discloses a brucella omp31 antigenic epitope and a monoclonal antibody thereof, and an application of the brucella omp31 antigenic epitope and the monoclonal antibody thereof. The sequence of the epitope is GGYIGINAGYAGGKFK. The invention also discloses the monoclonal antibody identifying the epitope, and the application of the epitope and the monoclonal antibody in preparing reagents or medicines used for diagnosing, preventing or treating brucellosis.

Description

technical field [0001] The invention relates to a brucella omp31 antigen epitope, and an anti-brucella omp31 protein monoclonal antibody stimulated by the epitope and application thereof. Background technique [0002] Brucellosis is one of the most prevalent zoonotic diseases in the world. According to the statistics of the World Health Organization, there are 500,000 cases of human brucellosis every year in the world, causing economic losses every year. up to $3 billion. The prevalence of brucellosis in my country is mainly the mixed infection of Brucella melis, which accounts for 80% of the epidemic strains. [0003] The clinical symptoms of brucellosis are complicated, and the pathological damage caused by fever and miscarriage is easy to be confused with some common diseases; it is difficult for doctors to diagnose brucellosis infection based on the history of contact with sick animals, which brings certain difficulties to the diagnosis. Difficult, easy to lead to misd...

Claims

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Application Information

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Patent Type & Authority Applications(China)
IPC IPC(8): C07K14/23C07K16/12A61K39/10A61K39/40A61P31/04G01N33/68G01N33/577G01N33/569C12N5/20
CPCC07K14/23A61K39/00A61K2039/505C07K16/1221G01N33/56911G01N2333/23
Inventor 王文敬吴静波廖卿宇黎诚耀
Owner SOUTHERN MEDICAL UNIVERSITY
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