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Brucella B cell epitope and monoclonal antibody and application thereof

A technology for brucellosis and brucellosis, applied in the direction of antibodies, bacterial antigen components, antibacterial drugs, etc., can solve the problem of unclear antigenic epitope, and achieve the effect of good specific recognition ability

Inactive Publication Date: 2013-07-03
SOUTHERN MEDICAL UNIVERSITY
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Problems solved by technology

[0006]Regarding the epitope research of bp26 protein, Patricia Seco-Mediavilla et al. constructed a series of expression plasmids for truncated polypeptides, and expressed and purified fusion proteins against bp26 monoclonal antibody Perform epitope screening, but the screened antigenic epitope segment is not clear

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  • Brucella B cell epitope and monoclonal antibody and application thereof
  • Brucella B cell epitope and monoclonal antibody and application thereof
  • Brucella B cell epitope and monoclonal antibody and application thereof

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Embodiment Construction

[0028] Below in conjunction with embodiment, further illustrate the present invention.

[0029] The Brucella M5-90 vaccine strain used in the present invention is purchased from Lanzhou Veterinary Research Institute and is a well-known vaccine strain in the industry.

[0030] Preparation of Recombinant Membrane Protein bp26 from Brucella

[0031] The constructed recombinant plasmid pET-28a-bp26 (gifted by Professor Chen Chuangfu, Shihezi University, Xinjiang) was transformed into competent BL21. After the recombinant bacteria were shaken to the logarithmic growth phase, IPTG was added at a final concentration of 1mM to induce the expression of the target protein. After the induction, it was sonicated, and it was identified that the target protein was mainly in the precipitate. After the inclusion bodies were washed with 5M urea inclusion body washing solution (1×PBS, 5M urea, 1% TritonX-100, 1mmol / L EDTA, pH8.0), the purity of the target protein was over 85%. The washed p...

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Abstract

The invention discloses a Brucella B cell epitope and a monoclonal antibody and application thereof. The sequence of the epitope is DRDLQTGGI. The Brucella B cell epitope of the invention is the core epitope of bp26 protein, in a vaccine strain, and the mutant bp26 protein obtained by mutation of this fragment of sequence cannot induce generation of antibodies against the epitope after being immunized in goat, so as to achieve differential diagnosis of natural infection and vaccine inoculation immunization.

Description

technical field [0001] The invention relates to a Brucella B cell epitope and an anti-brucella bp26 protein monoclonal antibody stimulated by the epitope. The invention also relates to the cell epitope antibody and the application of the cell epitope. Background technique [0002] Brucellosis (Brucellosis) is one of the most widespread zoonotic infectious diseases in the world. In the past ten years, the occurrence and prevalence of the disease have been on the rise worldwide. According to the statistics of the World Health Organization, there are 500,000 cases of human brucellosis every year in the world, and the annual economic loss is 3 billion US dollars. Accounted for 80% of the popular strains. [0003] Currently, there is no specific and effective treatment for brucellosis. Even long-term treatment with large doses of antibiotics still has limited effect. It is especially difficult to overcome the problem of bacterial strain resistance. Vaccination and removal of in...

Claims

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Application Information

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Patent Type & Authority Patents(China)
IPC IPC(8): C07K7/08C07K16/12G01N33/569G01N33/577A61K39/10A61K39/40A61P31/04
Inventor 王文敬丘金浪吴静波黎诚耀
Owner SOUTHERN MEDICAL UNIVERSITY
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