Brucella bp26 protein epitope, monoclonal antibody and application thereof
A monoclonal antibody, bp26 technology, applied in the direction of anti-bacterial immunoglobulin, antibody, bacterial antigen components, etc., can solve the problem of unclear antigenic epitope, and achieve the effect of good specific recognition ability
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[0028] Below in conjunction with embodiment, further illustrate the present invention.
[0029] The Brucella M5-90 vaccine strain used in the present invention is purchased from Lanzhou Veterinary Research Institute and is a well-known vaccine strain in the industry.
[0030] Preparation of Recombinant Membrane Protein bp26 from Brucella
[0031] The constructed recombinant plasmid pET-28a-bp26 (gifted by Professor Chen Chuangfu, Shihezi University, Xinjiang) was transformed into competent BL21. After the recombinant bacteria were shaken to the logarithmic growth phase, IPTG was added at a final concentration of 1mM to induce the expression of the target protein. After the induction, it was sonicated, and it was identified that the target protein was mainly in the precipitate. After the inclusion bodies were washed with 5M urea inclusion body washing solution (1×PBS, 5M urea, 1% TritonX-100, 1mmol / L EDTA, pH8.0), the purity of the target protein was over 85%. The washed p...
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