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Brucella bp26 protein epitope, monoclonal antibody and application thereof

A bp26, Brucella technology, applied in the direction of anti-bacterial immunoglobulin, antibody, bacterial antigen components, etc., can solve the problem of unclear antigenic epitope, and achieve the effect of good specific recognition ability

Inactive Publication Date: 2013-07-03
SOUTHERN MEDICAL UNIVERSITY
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  • Claims
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Problems solved by technology

[0006] Regarding the epitope research of bp26 protein, Patricia Seco-Mediavilla et al. constructed a series of expression plasmids for truncated polypeptides, and expressed and purified fusion proteins for epitope screening of anti-bp26 monoclonal antibodies, but the screened antigenic epitopes were not clear

Method used

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  • Brucella bp26 protein epitope, monoclonal antibody and application thereof
  • Brucella bp26 protein epitope, monoclonal antibody and application thereof
  • Brucella bp26 protein epitope, monoclonal antibody and application thereof

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Embodiment Construction

[0028] Below in conjunction with embodiment, further illustrate the present invention.

[0029] The Brucella M5-90 vaccine strain used in the present invention is purchased from Lanzhou Veterinary Research Institute and is a well-known vaccine strain in the industry.

[0030] Preparation of Recombinant Membrane Protein bp26 from Brucella

[0031] The constructed recombinant plasmid pET-28a-bp26 (gifted by Professor Chen Chuangfu, Shihezi University, Xinjiang) was transformed into competent BL21. After the recombinant bacteria were shaken to the logarithmic growth phase, IPTG was added at a final concentration of 1mM to induce the expression of the target protein. After the induction, it was sonicated, and it was identified that the target protein was mainly in the precipitate. After the inclusion bodies were washed with 5M urea concentration inclusion body washing solution (1×PBS, 5M urea, 1% TritonX-100, 1mmol / L EDTA, pH8.0), the purity of the target protein was over 85%....

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Abstract

The invention discloses a brucella bp26 protein epitope, a monoclonal antibody and application thereof. A sequence of the epitope is QPIYVYPD. The brucella bp26 protein epitope is a core epitope of bp26 protein; and the sequence in a vaccine plant is mutated to obtain mutant bp26 protein, and after being used for immunizing sheep, the protein cannot generate an antibody for resisting the epitope by induction, so that the aim of the differential diagnosis of natural infection and vaccinated immunization is fulfilled.

Description

technical field [0001] The invention relates to a Brucella bp26 protein epitope and an anti-brucella bp26 protein monoclonal antibody stimulated by the epitope. The invention also relates to the cell epitope antibody and the application of the cell epitope. Background technique [0002] Brucellosis (Brucellosis) is one of the most widespread zoonotic infectious diseases in the world. In the past ten years, the occurrence and prevalence of the disease have been on the rise worldwide. According to the statistics of the World Health Organization, there are 500,000 cases of human brucellosis every year in the world, and the annual economic loss is 3 billion US dollars. Accounted for 80% of the popular strains. [0003] Currently, there is no specific and effective treatment for brucellosis. Even long-term treatment with large doses of antibiotics still has limited effect. It is especially difficult to overcome the problem of bacterial strain resistance. Vaccination and removal...

Claims

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Application Information

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Patent Type & Authority Patents(China)
IPC IPC(8): C07K7/06G01N33/68A61K39/10A61P31/04C07K16/12G01N33/577A61K39/40
Inventor 王文敬丘金浪张玲黎诚耀
Owner SOUTHERN MEDICAL UNIVERSITY
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