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Extraction method of natural anthocyanidin active aglycone

An extraction method and technology for anthocyanin, applied in the field of bioengineering, can solve the problems of increasing the production cost of anthocyanin, wasting anthocyanin biological resources, unstable chemical structure, etc., so as to save raw materials, eliminate emissions, and reduce production costs Effect

Inactive Publication Date: 2014-09-24
耿兆翔
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  • Summary
  • Abstract
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  • Claims
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Problems solved by technology

However, because anthocyanins have strong biological activity and unstable chemical structure, they are only stable under low temperature / acid or dry conditions, and are easily decomposed under acidic heating conditions, and become water-insoluble substances and lose their biological activity. Degradation products (including microorganisms, enzymes and anthocyanins) are heat-sterilized at a temperature of 70°C-100°C, which will surely decompose and denature 10%-50% of anthocyanins into water-insoluble substances and lose their biological activity, wasting Extremely valuable anthocyanin biological resources increase the production cost of anthocyanin

Method used

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Experimental program
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Effect test

Embodiment

[0054] When extracting, proceed as follows:

[0055] 1. Pretreatment of raw materials: fresh raw materials containing plant anthocyanins, such as grape skins, carrots, and indigo fruits, are classified → pulverized → soaked in soft water to absorb water → frozen to destroy plant tissue cell walls → heated and thawed → squeezed → filtered to make juice for later use ( 1) → add water to the residue → soak and stir → squeeze → filter to make juice for later use (2);

[0056] Two, the above-mentioned squeezing and filtering aqueous solution (1) and (2) are put together, and its water temperature is adjusted to the most suitable scope of following degrading biological enzyme;

[0057] 3. Biodegrade the above product with β-glucosidase (β-glucosidase) that can crack the glycosidic bond of anthocyanin, first stir at a low speed, 50 rpm, and then let stand to degrade the glycosidic bond in the anthocyanin To obtain cationic anthocyanin active aglycone; the cracking and degradation ti...

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Abstract

The invention relates to an extraction method of natural anthocyanidin active aglycone, which belongs to the technical field of bioengineering. The method comprises the following steps: performing classification, crushing, water absorption, freezing, heating for unfreezing, and filtering for juice preparation of anthocyanidin-containing plants, performing biodegradation of anthocyanin by glycosidase for decomposing anthocyanin glucosidic bonds, performing combination reaction precipitation / suspension by an anionic surfactant, degrading the precipitates / suspensions by bacterial microbe, and performing sterilization, enzyme and bacterium filtration, and freeze drying of the anthocyanidin aqueous solution. The method of the invention can obtain cationic anthocyanidin, instead of anthocyanin, with a real content of more than 95%. The cationic anthocyanidin prepared by the method of the invention needs no degradation by microbe in human body intestinal tracts, can be absorbed and used directly by human body, or can directly enter human body blood, and is applicable to medicine and health care.

Description

Technical field: [0001] The invention relates to a method for extracting plant cationic anthocyanins in the technical field of bioengineering, in particular to a method for extracting active aglycones of natural anthocyanins. Background technique: [0002] With the improvement of people's living standards and the pursuit of non-toxic and harmless natural organic substances from natural plant sources that are beneficial to protect people's health, prevent and treat diseases, and because artificial synthetic pigments are relatively toxic to the human body Side effects have been banned by the World Health Organization, and because of the large investment in anthocyanin industrial synthesis technology, complex and unstable technology, and poor product quality, it cannot serve human beings. Therefore, efficient and non-toxic natural cationic anthocyanin active aglycones and their extraction methods have become emerging frontier research topics in the 21st century. [0003] Howev...

Claims

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Application Information

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IPC IPC(8): C12P17/06
Inventor 耿兆翔
Owner 耿兆翔
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