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Onychium japonicum extract with anti-inflammatory action and preparation method thereof

A technology of anti-inflammatory and detoxifying herbs, applied in anti-inflammatory agents, drug combinations, medical raw materials derived from ferns/filamentous plants, etc.

Inactive Publication Date: 2014-09-24
广西壮族自治区民族医药研究院(广西壮医医院)
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

At present, there is no research report on the preparation method of the total flavonoids extract of Jieducao and its anti-inflammatory effect.

Method used

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Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0012] Cotton balls induce granuloma in rats

[0013] Method: Take 50 SD rats, half male and half male, and divide them into 5 groups randomly. That is, NS control group, positive control group (aspirin 200mg.kg-1) and Jieducao total flavonoids extract group were intragastrically administered once a day for 5 consecutive days, and the model group was given corresponding volume of normal saline. After the last gavage, 0.1 ml of 1% carrageenan was subcutaneously injected into the right toe of each mouse to induce inflammation. Before the inflammation and 1, 3, 5, and 7 hours after the inflammation, the volume of the inflamed lateral paw was measured with a paw meter, and the swelling degree was defined as the difference between the volumes before and after the inflammation of each mouse.

[0014] Experimental conclusion: The two doses of total flavonoids extract of Jieducao 4g / kg and 8mg / kg can significantly inhibit the paw swelling caused by carrageenan at each time period.

Embodiment 2

[0016] Determination of Biochemical Indexes in Blood and Paw Tissues of Rats

[0017] After the paw volume measurement of the above-mentioned rats was completed, blood was taken from the femoral artery, centrifuged (3000r / min, 5min), and the serum was stored at -20°C for each biochemical index to be tested. The soles of the paws were removed from the left ankle of the rats, peeled, weighed and cut into pieces, soaked in 20% saline for 40 minutes, and the supernatant was stored at -20°C to store the biochemical indicators to be tested.

[0018] A. Determination of the amount of malondialdehyde (MDA) Take 0.5ml of tissue soaking solution or 01ml of serum sample and add it to 2.5ml of 25% trichloroacetic acid, shake well and then add 0.67% thiobarbituric acid (TBA) 1ml, heated in boiling water for 30min, cooled in running water, 30000r×10min, centrifuged to take the supernatant and measured the absorbance A value at 535nm, with 2μmol / L tetraoxymethylpropane (TMP) solution as the ...

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PUM

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Abstract

The invention relates to the field of formulas for plant Chinese herbal medicines, specifically to an Onychium japonicum extract with anti-inflammatory action and a preparation method thereof. The preparation method for the Onychium japonicum extract comprises the following steps: selecting the medicinal material Onychium japonicum, carrying out extraction by using water or an ethanol-water mixed solvent with a concentration of 40 to 80%, carrying out concentration so as to obtain Onychium japonicum concentrate, adding a proper amount of water to dilute the Onychium japonicum concentrate, then carrying out standing at normal temperature for 6 to 24 h and carrying out filtering so as to obtain an Onychium japonicum solution; and adsorbing the prepared Onychium japonicum solution by using a pretreated non-polar or weakly polar macroporous resin, carrying out rinsing, then carrying out elution with the ethanol-water mixed solvent, wherein the concentrations of ethanol are 15 to 35% and 40 to 90%, successively, collecting eluate at an elution part where ethanol with a concentration of 40 to 90% is used, carrying out pressure reduction to recover the solvent and then successively carrying out concentration and drying so as to obtain Onychium japonicum general flavone extract. The Onychium japonicum extract has good anti-inflammatory and pain-easing effects and exerts good effects on writhing reaction of mice induced by acetic acid; and the preparation method has the advantages of reduced purification cost, easy realization of industrial production and no pollution to the environment.

Description

Technical field: [0001] The invention relates to the field of plant Chinese herbal medicine formulations, in particular to an anti-inflammatory herb extract and a preparation method thereof. technical background: [0002] Jiedu herb is the whole herb of Onychium japonicum (Thunb.) Kunze, a Chinese fern plant, also known as small-leaf golden flower grass, human head grass, small golden flower grass, and Jiedu fern. It is a commonly used drug for ethnic minorities in Southwest China. It is bitter in taste and cold in nature, and has the effects of clearing heat, dampness, detoxification, and hemostasis. It is used for wind-heat cold, cough, acute gastroenteritis, dysentery, jaundice, hemoptysis, vomiting blood, blood in stool, hematuria, pesticide poisoning, arsenic poisoning , Food poisoning, cassava poisoning, wild yam poisoning, boils, burns, trauma swelling and pain embolism. The whole herb of detoxification grass mainly contains flavonoids fern, fernin, gold fernol, kae...

Claims

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Application Information

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IPC IPC(8): A61K36/11A61P29/00
Inventor 滕红丽郭力城
Owner 广西壮族自治区民族医药研究院(广西壮医医院)
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