A method for breeding a three-line sterile line of hybrid rice with white midribs on leaves
A three-line male sterile line and hybrid rice technology, applied in the field of hybrid rice three-line male sterile line breeding, can solve the problem of reducing rice photosynthetic efficiency, affecting the growth period of rice male sterile line, agronomic traits, and greatly affecting rice photosynthesis and other problems, to achieve the effects of good plant and leaf morphology, excellent agronomic traits, and improvement of the purity of sterile lines and hybrids
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Embodiment 1
[0029] Example 1 Breeding of Rice Carrying Leaf Midrib as White Mark
[0030] (1) Get 3000 dry seeds of "Ningjing No. 3" with a moisture content of 13%, carry out cobalt-60 gamma ray mutagenesis, dose 250Gy, dose rate 10Gy / min, get mutagenic seeds (M 0 ).
[0031] (2) direct seeding mutation seeds, mixed with the seeds of all mutation plants (M 1 ).
[0032] (3) The seeds obtained in step (2) are planted and transplanted by individual plants, and 100,000 individual plants are planted in the field, and 5 mutant individual plants (M 2 ).
[0033] (4) The above-mentioned 5 variation single plants are planted by the single plant of the strain, observe the stability of the midrib of the leaf between the strain and the single plant for the white trait, and screen 4 stable strains (M 3 ).
[0034] (5) Evaluate the expression of the white midrib of the leaves at different development stages, and screen the lines whose midrib is expressed in the whole growth period of the white tr...
Embodiment 2
[0043] Example 2 Breeding of Hybrid Rice CMS Line and Maintainer Line Carrying Leaf Midrib as White Mark
[0044]Breeding material:
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