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Scale production technology of high-concentration liquid nitrobacteria

A nitrifying bacteria, high-concentration technology, applied in bacteria, chemical instruments and methods, biological water/sewage treatment, etc., can solve the problems of productization, slow commercialization process, and the large-scale cultivation and preservation technology of nitrifying bacteria has not been overcome. To achieve the effect of long storage time, vigorous growth of live bacteria, and large continuous collection

Inactive Publication Date: 2014-09-17
都江堰惠农生物技术有限责任公司
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Problems solved by technology

Therefore, the large-scale cultivation and preservation technology of nitrifying bacteria has not been overcome, and its productization and commercialization have been progressing very slowly.

Method used

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Embodiment Construction

[0014] The present invention will be described in further detail below in conjunction with embodiment:

[0015] The specific operation process is as follows:

[0016] (1) Prepare the aerobic fermenter. A large-scale plastic pool with a depth of about 1 meter is selected, and air pipes are evenly arranged at the bottom, and the air is supplied by an air pump. The gas passes through an air filtration system to minimize bacteria and impurities in the air. Ensure that the dissolved oxygen in the culture medium is not less than 6mg / l. Fill with 4 / 5 volume of distilled water.

[0017] (2) Prepare the non-woven fabric. The material is a soft, porous and dense thin-layer chemical fiber (PVC fiber cotton) with a specification of 30X10X3cm (length X width X thickness), which is evenly arranged in the water body of the fermenter. The basic parameters of each carrier are measured as follows: geometric surface area 840cm2, specific gravity 0.8±0.1kgnf3, specific surface area ≥ 93.3m2m...

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Abstract

The invention discloses a scale production technology of high-concentration liquid nitrobacteria. The scale production technology comprises the following steps of arranging soft fibers in an aerobic fermenter, promoting nitrobacterium adhesion and growth by a low-molecular organic carbon source-containing medium, regularly supplying a low-molecular organic carbon source and nitrogen source, regularly eluting nitrobacteria on the soft fibers, storing the collected liquid under the conditions of low-dissolved oxygen, low-pH value and seal environment, carrying out detection, and carrying out packaging by a package vessel. The high-concentration liquid nitrobacteria obtained by the scale production technology can grow exuberantly and have a large continuous-collection amount, simple preservation conditions and long preservation time. The scale production technology can realize productization and commercialization of nitrobacteria (liquid). In actual use, the nitrobacteria are directly spayed to water and thus after a certain time, bioremediation of culture water environment and landscape water environment can be realized.

Description

technical field [0001] The invention relates to a large-scale production process of high-concentration liquid nitrifying bacteria. The product is a microbial preparation (liquid) mainly used to improve the water environment and landscape water environment. Background technique [0002] There are mainly two types of nitrifying bacteria in the water environment: Nitrosomonas and Nitrobacter, which can efficiently convert ammonia nitrogen and nitrite to nitrate, so they have been widely valued. Nitrifying bacteria are mainly used in two aspects. One is to relieve the toxic effects of ammonia nitrogen and nitrite on farmed animals and ornamental animals; the other is to perform nitrification (or nitrification process), which is conducive to the next step of denitrification process and effectively reduces the water body. Nitrogen load. Nitrifying bacteria are typical chemoautotrophic bacteria, which obtain energy from the process of biological oxidation of ammonia nitrogen to n...

Claims

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Application Information

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Patent Type & Authority Applications(China)
IPC IPC(8): C12N1/20C02F3/34
Inventor 李朝均
Owner 都江堰惠农生物技术有限责任公司
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