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A culture medium for promoting the differentiation of rat neural stem cells and its application method

A neural stem cell and culture medium technology, applied in the field of neural stem cell culture and differentiation, can solve the problems of low neural stem cell differentiation efficiency and other problems

Active Publication Date: 2016-01-13
CENT SOUTH UNIV
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Problems solved by technology

[0004] The purpose of the present invention is to provide a culture medium for promoting the differentiation of rat neural stem cells and its use method, to solve the problem of low differentiation efficiency of the existing neural stem cells, and to improve the efficiency of the differentiation of neural stem cells into neurons

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  • A culture medium for promoting the differentiation of rat neural stem cells and its application method
  • A culture medium for promoting the differentiation of rat neural stem cells and its application method
  • A culture medium for promoting the differentiation of rat neural stem cells and its application method

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Embodiment Construction

[0015] The following in conjunction with specific embodiments is intended to further illustrate the present invention, rather than limit the present invention.

[0016] 1) Preparation of complete medium for neural stem cells:

[0017] 100ml Neurobasal-Amedium neural stem cell special medium contains the following components:

[0018] B-27SupplementB27 additive 2ml (final concentration 2% volume ratio);

[0019] L-Glutamine glutamine (mother solution concentration is 200mM) 1ml (final concentration 2mM);

[0020] EGF epidermal growth factor (mother solution concentration is 100 μg / ml) 20 μl (final concentration 50ng / ml);

[0021] bFGF basic fibroblast growth factor (concentration of mother solution is 25 μg / ml) 80 μl (final concentration 50ng / ml);

[0022] Heparin sodium (concentration of mother solution is 5 mg / ml) 100 μl (final concentration 50 μg / ml);

[0023] Prepare the mother solution first, store it at -20 degrees, and then add it to the final concentration each time...

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Abstract

The invention discloses a culture medium for promoting differentiation of rat neural stem cells and a use method of the culture medium. The culture medium contains B-27 Supplement without retinoic acid, L-Glutamine, FBS (fetal bovine serum), beta-NGF (neural growth factor), BDNF (brain derived neurotrophic factor), RA and vitamin E in Neurobasal-A medium. The culture medium is low in cost, the problem of low neural stem cell differentiation efficiency in the prior art is solved, and the efficiency of differentiating neural stem cells into nerve cells is remarkably improved.

Description

technical field [0001] The invention belongs to the technical field of neural stem cell culture and differentiation, and in particular relates to a culture medium for promoting the differentiation of rat neural stem cells and a use method thereof. Background technique [0002] Traditional views generally believe that the self-repair ability of the central nervous system is limited, and neurons after injury will not be able to regenerate. Nevertheless, scholars all over the world are trying to challenge this traditional concept. Since Reynolds and Weiss isolated and cultured neural stem cells from striatal tissue of mice in the 1990s, people have not only isolated and cultured neural stem cells from embryos of various animals and humans, but also from central nervous system tissues of adult animals and humans. Neural stem cells have been cultured and proved to have strong proliferation and self-renewal capabilities, breaking this traditional concept. The academic circles cu...

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Application Information

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Patent Type & Authority Patents(China)
IPC IPC(8): C12N5/0797
Inventor 李志远邓思浩薛志琴贺旭钟小林胡杏
Owner CENT SOUTH UNIV
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