Large-scale seedling culturing technology for rhododendron calophytum Franch

A kind of Rhododendron technology, applied in the field of seedling raising technology, can solve the problems such as disinfection and sterilization of explants difficult to propagate by tissue culture, low survival rate of seedlings, infeasible propagation by cuttings and propagation by grafting, etc.

Inactive Publication Date: 2014-09-03
赵冰
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Problems solved by technology

But its introduction and propagation is very difficult, because of its high growth altitude, so the survival rate of seedlings introduced directly is very low, cutting propagation and grafting propagation are also almost infeasible, tissue culture propagation is more difficult to solve the problem of explant disinfection and sterilization, so Seed propagation has become the only way to reproduce in large quantities and protect its resources, and it can also be used as a method for seedling breeding of this species
The existing rhododendrons are sown and propag...

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  • Large-scale seedling culturing technology for rhododendron calophytum Franch
  • Large-scale seedling culturing technology for rhododendron calophytum Franch
  • Large-scale seedling culturing technology for rhododendron calophytum Franch

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[0015] In order to make the objects and advantages of the present invention clearer, the present invention will be further described in detail below in conjunction with the examples. It should be understood that the specific embodiments described here are only used to explain the present invention, not to limit the present invention.

[0016] This embodiment provides a large-scale seedling raising technique for beauty rhododendron, comprising the following steps:

[0017] S1. Put the beauty rhododendron seeds collected in the previous year in the refrigerator, and store them at a low temperature of 4 degrees until March of the next year;

[0018] S2, the seeds obtained in step S1 were treated with 600 mg / l 6A3 for 24 hours, and then washed with running water for 10 minutes;

[0019] S3. Select mature and plump seeds and put them into a petri dish that is lined with degreasing gauze and filter paper, place them in an incubator with a low temperature of 20°C and 16h / 8h (light / d...

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Abstract

The invention discloses a large-scale seedling culturing technology for rhododendron calophytum Franch. The technology includes the following steps that seeds, collected in the last year, of the rhododendron calophytum Franch are placed in a refrigerator and stored to March in the second year at the temperature of 4 DEG C; the seeds are treated for 24 hours through 600 mg/l GA3 and then flushed for 10 minutes through running water; the mature and full seeds are selected and placed in a culture dish where absorbent gauze and filter paper are arranged in a cushioning mode, then the culture dish is placed in a culture box for culturing, wherein the culture box is at the low temperature of 20 DEG C, illumination is performed according to 16 h/8 h (light/dark), water is sprayed one time per day, and the rate of emergence of the seedlings can reach over 95% after two weeks; all the emerged seedlings are transplanted into a plastic green house and placed in matrixes for planting, wherein peat and perlite are mixed in the substrates, and then water is sprayed every day. A lot of rhododendron calophytum Franch plants are obtained; the resources of wild rhododendron calophytum Franch in Qinling mountains are conserved and protected; the large-scale seedling culturing technology lays the foundation for seedling culturing of the rhododendron calophytum Franch; material stock is provided for hybridization seed culturing of existing monotonous rhododendron varieties in China.

Description

technical field [0001] The invention relates to a seedling raising technology, in particular to a large-scale seedling raising technology of rhododendron beautifense. Background technique [0002] Rhododendron calophytum Franch. is an evergreen shrub of Rhododendron calophytum Franch. Among the few Rhododendron plants in the Qinling Mountains, it has high ornamental value and breeding value because of its huge inflorescence, rich variation and early flowering period. But its introduction and propagation is very difficult, because of its high growth altitude, so the survival rate of seedlings introduced directly is very low, cutting propagation and grafting propagation are also almost infeasible, tissue culture propagation is more difficult to solve the problem of explant disinfection and sterilization, so Seed propagation has become the only way for it to reproduce in large quantities and protect its resources, and it can also be used as a method for seedling breeding of th...

Claims

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Application Information

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IPC IPC(8): A01G31/00A01C1/08
Inventor 赵冰张冬林董进英郑茜子李厚华
Owner 赵冰
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