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Maize salt tolerance identification method

An identification method and salt tolerance technology, applied in the field of plant physiology, can solve the problems of plant growth retardation, leaf wilting, leaf top whitening, etc., and achieve the effect of avoiding human operation errors, long identification period and short experiment period

Inactive Publication Date: 2014-09-03
INST OF CROP SCI CHINESE ACAD OF AGRI SCI
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

[0004] As the world's largest food crop, corn is of great significance to world food security, and corn is a salt-sensitive crop. Once corn is subjected to salt stress, it will show leaf wilting, leaf top albinism, plant growth retardation, and biological Production declines, which eventually lead to severe production cuts

Method used

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Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0025] The cultivation of embodiment 1 corn seedling and salt stress treatment

[0026] 1. Material preparation: Collect seeds of 9 corn inbred lines from different corn materials, including CZ-1, CZ-7, CZ-10, BM-7, Bornali, Shuvra, Mohor, and Khoi Bhutta from Bangladesh Agricultural Research Institute, B73 provided by the Institute of Crop Science, Chinese Academy of Agricultural Sciences.

[0027] 2. Germination of seedlings: Rinse the above seeds with deionized water 3 times, place them on germination paper, and germinate them in an incubator at 28°C for 48 hours. Each material has 3 treatments, and each treatment includes 3 repetitions. Repeat planting 4 plants.

[0028]3. Cultivation conditions: Transplant the seeds that germinate uniformly into small flowerpots filled with vermiculite. After all the seedlings are sown, place the flowerpots in the incubator. The cultivation conditions are: 25°C for 16 hours, light intensity 450 μmolm-2s-1, 23 ° C dark for 8 hours, relat...

Embodiment 2

[0030] Embodiment 2 above-ground part fresh weight and dry weight weighing

[0031] 1. Weighing the fresh weight of the above-ground part: after about 4 weeks of stress treatment of the seedlings, the seedlings of some materials show wilting phenotype, take the seedlings out of the flower pot, cut off the above-ground part along the root, and quickly weigh the weight, which is the above-ground part Fresh weight.

[0032] 2. Weighing the fresh weight of the above-ground part: place the fresh-weighted seedlings in a paper bag, place them in an oven at 85°C, and dry them continuously for 48 hours, then weigh the dry weight, that is, the dry weight of the above-ground parts ( figure 2 and image 3 ).

Embodiment 3

[0033] Na in the sample of Example 3 + 、K + Determination of the ion content of

[0034] 1. Sample weighing: weigh the dried sample on a balance and put it into a volumetric flask.

[0035] 2. Sample digestion: add 10 times the volume of the sample to the above sample into the digestion solution (HNO 3 :HClO 4＝ 4:1, volume ratio), pre-digested for 12 hours, put the beaker containing the digest in a horse-boiling furnace, and heated at 175°C for 2-3 hours until the solution became clear and transparent.

[0036] 3. Sample dilution: add deionized water to the digestion solution, transfer it to a test tube and dilute to 25mL, absorb 1mL and dilute to 15mL with deionized water after mixing, then take 1mL and dilute to 10mL after mixing.

[0037] 4. Na + 、K + Ion determination: place the diluted solution in an atomic absorption spectrometer and measure Na + 、K + Ion content, the digestion solution without sample was used as the blank control.

[0038] Na + 、K + Ion conte...

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Abstract

The invention provides a maize salt tolerance identification method. According to the method, the salt tolerance of materials is evaluated by comparing the fresh weights of the overground parts, the dry weights of the overground parts and the K+ / Na+ ion content ratios of different materials which are subjected to salt stress. The experiment period is short, one month is enough from the start of treatment to the end of investigation, and the problem of long period of field identification is avoided. Operation is easy, identification indices only include material weights and ion contents, and the method is suitable for large-scale maize material identification. Furthermore, the fresh weights of the overground parts, the dry weights of the overground parts and the K+ / Na+ ion content ratios are adopted as the identification indices to conduct mutual verification with the overground part dry weight and fresh weight indices, and manual operating errors are avoided. The method has the advantages of being accurate and reliable.

Description

technical field [0001] The invention relates to plant physiology, in particular to a method for identifying salt tolerance of corn. Background technique [0002] Soil salinization, as a major abiotic stress, seriously affects crop yields worldwide. The total global area of ​​saline soil in the world is 8.3 million hectares, about 20% of which belong to irrigated farmland, and the salinization has a tendency to further intensify. As the world's total population continues to grow, especially in some underdeveloped countries and some newly industrialized countries, the contradiction between population growth and the reduction of available arable land is increasingly intensified. Therefore, it is particularly important to improve the salt tolerance of crops. [0003] The main effects of salinity on crops are: osmotic stress, ion toxicity and ion metabolism disorder. In salinized soil, plants absorb Na through water diversion in the root system + 、K + Salt ions, these ions a...

Claims

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Application Information

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IPC IPC(8): A01G1/00A01G7/00
Inventor 郑军陈勋基王国英
Owner INST OF CROP SCI CHINESE ACAD OF AGRI SCI
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