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Method for promoting seeds of meconopsis integrifolia to germinate

A technology for M. chinensis and seeds is applied in the directions of seed and rhizome treatment, application, agriculture, etc., which can solve the problems of artificial cultivation and breeding methods that are rarely reported and no seed germination, etc., and achieves maintenance of genetic diversity and expansion. The effect of population and protection of wild resources

Active Publication Date: 2014-09-03
GANSU UNIV OF CHINESE MEDICINE
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

[0011] However, due to the special environment for the growth of Meconopsis entire margin seeds, there is no effective means to rapidly promote its seed germination, and there are few reports on its artificial cultivation and breeding methods.

Method used

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Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0031] A method for promoting seed germination of Meconopsis whole margin, comprising the following steps:

[0032] (1) Bake 200 grains of Meconopsis whole-leaf seeds at 30°C for 15 hours;

[0033] (2) Place the seeds baked in step (1) at -15°C, with a humidity of 70%, and in the dark for 48 hours;

[0034] (3) Place the seeds obtained in step (2) in a polyethylene glycol 2000 solution with a mass concentration of 12%, keep the temperature at 6°C, and soak for 2 days in the dark;

[0035] (4) Soak the seeds soaked in step (3) in 10wt.% ammonia water for 2 hours, and then clean the surface of the seeds with distilled water;

[0036] (5) Soak the cleaned seeds in step (4) in 50mg / L gibberellin solution for 12 hours, and then clean the surface of the seeds with distilled water;

[0037] (6) Place the cleaned seeds in step (5) in seed germination boxes (15cm*15cm), 50 seeds in each germination box, and germinate at 15°C, 90% humidity, and dark conditions.

Embodiment 2

[0039] A method for promoting seed germination of Meconopsis whole margin, comprising the following steps:

[0040] (1) Bake 200 grains of Meconopsis whole-leaf seeds at 36°C for 13 hours;

[0041] (2) Place the seeds baked in step (1) at -13°C, with a humidity of 75%, and in the dark for 24 hours;

[0042] (3) Place the seeds obtained in step (2) in a polyethylene glycol 2000 solution with a mass concentration of 16%, keep the temperature at 2°C, and soak for 2 days in the dark;

[0043] (4) Soak the seeds soaked in step (3) in 10wt.% ammonia water for 4 hours, and then clean the surface of the seeds with distilled water;

[0044] (5) Soak the cleaned seeds in step (4) in 250mg / L gibberellin solution for 18 hours, and then clean the surface of the seeds with distilled water;

[0045] (6) Place the cleaned seeds in step (5) in seed germination boxes (15cm*15cm), 50 seeds in each germination box, and germinate at 13°C, 80% humidity, and dark conditions.

Embodiment 3

[0047] A method for promoting seed germination of Meconopsis whole margin, comprising the following steps:

[0048] (1) Bake 200 grains of Meconopsis whole-leaf seeds at 35°C for 12 hours;

[0049] (2) Place the seeds baked in step (1) at -12°C, with a humidity of 80%, and in the dark for 40 hours;

[0050] (3) Place the seeds obtained in step (2) in a polyethylene glycol 2000 solution with a mass concentration of 15%, keep the temperature at 5°C, and soak for 3 days in the dark;

[0051] (4) Soak the seeds soaked in step (3) in 10wt.% ammonia water for 3 hours, and then clean the surface of the seeds with distilled water;

[0052] (5) Soak the cleaned seeds in step (4) in 100mg / L gibberellin solution for 15 hours, and then clean the surface of the seeds with distilled water;

[0053](6) Place the cleaned seeds in step (5) in seed germination boxes (15cm*15cm), 50 seeds in each germination box, and germinate at 10°C, humidity 85%, and dark conditions.

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Abstract

The invention discloses a method for promoting seeds of meconopsis integrifolia to germinate. The method comprises the steps of firstly drying the seeds of the meconopsis integrifolia for 10-15 hours at 30-40 DEG C; then putting the dried seeds in darkness for 24-48 hours at the conditions that the temperature is -15--10 DEG C and the humidity is 70-80%; putting the seeds into a polyethylene glycol solution with the mass concentration being 12-16%, keeping at 2-6 DEG C, and soaking for 15-24 hours in darkness; soaking for 2-4 hours in 10wt% ammonia water, and washing the surfaces of the seeds by distilled water; soaking the seeds in 50-400mg / L gibberellin solution for treating for 12-18 hours, and washing the surfaces of the seeds by the distilled water; finally, germinating in the darkness under the conditions that the temperature is 5-10 DEG C and the humidity is 80-90%. According to the method, the germination rate of the seeds reaches over 87% on the 20th day.

Description

technical field [0001] The invention belongs to the field of seed germination, and in particular relates to a method for promoting the germination of Meconopsis whole-leaf seeds. Background technique [0002] Meconopsis integrifolia (Maxim.) Franch is an annual to perennial herb, produced in the Qinghai-Tibet Plateau and surrounding areas, mainly growing in alpine meadows and shrubs at an altitude of 3200-3800m. It is used as a classic Tibetan medicine "Wuba It has the effects of clearing away heat and detoxification, reducing inflammation and relieving pain, and is used to treat pneumonia, hepatitis, headache, edema and other diseases. [0003] In recent years, due to ecological damage, community degradation and other reasons, wild resources are on the verge of extinction, and artificial planting is the only way to meet their medicinal needs. Meconopsis entire margins are mainly propagated by seeds. According to "Journal of Northwest Botany" No. 9, 2008, Shi Huizhen, Liu M...

Claims

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Application Information

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IPC IPC(8): A01C1/00
Inventor 陈红刚杜弢杨韬高素芳
Owner GANSU UNIV OF CHINESE MEDICINE
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