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Method for production of bacillus subtilis preparation by utilizing ethanol industrial yellow water

A technology of Bacillus subtilis and alcohol industrial yellow water, applied in the direction of microorganism-based methods, biochemical equipment and methods, bacteria, etc., can solve the problems of increasing costs, affecting the application of Bacillus subtilis, and high production costs, so as to increase production, The effect of improving comprehensive utilization value and reducing production cost

Inactive Publication Date: 2014-08-27
HENAN UNIV OF SCI & TECH
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

However, the current production methods of Bacillus subtilis preparations mostly use artificially adding a large amount of high-quality nutrients as raw materials for production, and its production cost is relatively high, which in turn affects the application of Bacillus subtilis and increases the cost of related products that use Bacillus subtilis as raw materials

Method used

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  • Method for production of bacillus subtilis preparation by utilizing ethanol industrial yellow water
  • Method for production of bacillus subtilis preparation by utilizing ethanol industrial yellow water
  • Method for production of bacillus subtilis preparation by utilizing ethanol industrial yellow water

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Effect test

Embodiment 1

[0030] Embodiment 1: a kind of method utilizing the yellow water of alcohol industry to produce subtilis preparation, what this method adopted is that corn is the yellow water of raw material production alcohol, and concrete steps are as follows:

[0031] (1) Collect alcohol industrial yellow water directly from the plate and frame of the plate and frame filter press, quickly put it into a sealed container, and then take samples to determine the content of nutrients such as protein, cellulose, amino acids, organic acids, and mineral elements, and obtain The content of various nutrients in the yellow water of the alcohol industry, the measurement results are shown in the attached figure 1 , and according to the nutritional needs of the actual measured value and the growth and reproduction of Bacillus subtilis, nutritional supplements are carried out. The types and quantities of specific nutritional supplements are: add urea 5g, glucose 2g, sodium chloride 2g and 3g corn flour; ...

Embodiment 2

[0041] Embodiment 2: a kind of method utilizing the yellow water of alcohol industry to produce Bacillus subtilis preparation, what this method adopted is that corn is the yellow water of raw material production alcohol, and concrete steps are as follows:

[0042] (1) Collect alcohol industrial yellow water directly from the plate and frame of the plate and frame filter press, quickly put it into a sealed container, and then take samples to determine the content of nutrients such as protein, cellulose, amino acids, organic acids, and mineral elements, and obtain The content of various nutrients in the yellow water of the alcohol industry, the measurement results are shown in the attached figure 1 , and according to the nutritional needs of the actual measured value and the growth and reproduction of Bacillus subtilis, the nutritional components are supplemented. The specific nutritional components supplemented type and quantity are: add urea 5g, glucose 2g, sodium chloride 2g a...

Embodiment 3

[0052] Embodiment 3: a kind of method utilizing alcohol industrial yellow water to produce Bacillus subtilis preparation, what this method adopted is that corn is the yellow water of raw material production alcohol, and concrete steps are as follows:

[0053] (1) Collect alcohol industrial yellow water directly from the plate and frame of the plate and frame filter press, quickly put it into a sealed container, and then take samples to determine the content of nutrients such as protein, cellulose, amino acids, organic acids, and mineral elements, and obtain The content of various nutrients in the yellow water of the alcohol industry, the measurement results are shown in the attached figure 1 , and according to the nutritional needs of the actual measured value and the growth and reproduction of Bacillus subtilis, the nutritional components are supplemented. The specific nutritional components supplemented type and quantity are: add urea 5g, glucose 2g, sodium chloride 2g and 3...

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Abstract

A method for production of a bacillus subtilis preparation by utilizing ethanol industrial yellow water includes collection and treatment of the ethanol industrial yellow water, cultivation of bacillus subtilis in the ethanol industrial yellow water, and receiving and preparation of the bacillus subtilis. The method for production of the bacillus subtilis by utilizing the ethanol industrial yellow water not only can make full use of the ethanol industrial yellow water, reduce the treatment cost of the yellow water, optimize comprehensive utilization of the yellow water and improve the ecological environment, but also can expand sources of a nutrition substrate in a conventional bacillus subtilis preparation production, lower the production costs and expand the application scope of bacillus subtilis.

Description

technical field [0001] The invention relates to the field of comprehensive utilization of yellow water in the alcohol industry and the field of microbial fermentation, in particular to the use of yellow water in the alcohol industry for microbial fermentation. Background technique [0002] Alcohol industry is an important energy industry in my country, but huge by-products will be produced in the process of alcohol production, among which solid components such as distiller's grains and part of yellow water can continue to produce feed materials such as DDGS and be reused, and most of them are The yellow water has not been well utilized. Yellow water is a liquid substance rich in various nutrients discharged during the production of alcohol. Yellow water is rich in organic acids (especially carboxylic acids such as lactic acid, butyric acid, caproic acid, and acetic acid), alcohols, aldehydes, At the same time, it also contains amino acids, sugars, alcohol, humus, yeast autoly...

Claims

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Application Information

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IPC IPC(8): C12N1/20C12R1/125
Inventor 何万领丁轲李晓丽李旺李元晓张广勤
Owner HENAN UNIV OF SCI & TECH
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