Oil-gas exploration method based on anomaly of light hydrocarbon oxidizing bacteria
A technology of oxidizing bacteria and methane oxidizing bacteria, applied in the field of oil and gas microbial exploration, can solve problems such as false positives, reduce the impact of false positives, reduce the risk of false positive results, and avoid cumbersome operations
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[0022] The present invention comprises the following steps:
[0023] a. Soil sampling in the oil field development area or undeveloped area, the soil sampling point density is 100 m × 100 m ~ 1000 m × 1000 m grid sampling, the sampling depth is 20 cm ~ 250 cm, and the sampling volume is 200 g ~ 500 g g, this embodiment adopts a grid sampling point density of 250 m×250 m, and the sampling depth of this embodiment is 50 cm;
[0024] b. After homogenizing the soil samples obtained at the above sampling points with a grinding bead homogenizer produced by Biospec Company of the United States, DNA extraction was carried out using the Powerful Soil DNA Extraction Kit of Mobio Company of the United States.
[0025] c. Use the stepone fluorescent quantitative PCR instrument produced by the American ABI company to perform real-time fluorescent quantitative PCR detection of the propane monooxygenase prmA gene in the sample, and the measured value can represent the number of propane-oxidi...
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