Method for ultra low temperature storage of Saussurea involucrata Kar.et Kir callus

A technology of ultra-low temperature preservation and callus, which is applied in horticultural methods, plant preservation, botanical equipment and methods, etc., and can solve the problems of germplasm resource preservation limitations, a lot of manpower and material resources, etc.

Inactive Publication Date: 2014-08-13
NORTHWEST UNIV(CN)
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

[0004] The conventional preservation of plant germplasm resources is mainly in the form of field planting and indoor storage, which requires a lot of manpower and material resources, which limits its wide application in the preservation of germplasm resources.

Method used

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Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0022] 1. Obtaining Xinjiang Snow Lotus Callus

[0023] Xinjiang snow lotus seeds were taken, sterilized with 0.1% mercury chloride, and then transferred to MS medium for germination. The cotyledons grown after germination were used as explants and cut into 0.3cm 2 size, and then cultured on MS medium containing 2.5mg / L a-naphthyl acetic acid and 0.5mg / L 6-benzylaminoadenine, the culture temperature was 25°C±2°C, the light intensity was 3000Lux, and the photoperiod was 16 hours; After 35 days of culturing, callus of Saussurea sauraceae was obtained;

[0024] 2. The subculture of Xinjiang Saussurea chinensis callus

[0025] The Xinjiang Saussurea sauvignon callus induced in step 1 was transferred to MS medium containing α-naphthaleneacetic acid: 2.5mg / L, 6-benzylaminoadenine: 0.5mg / L for cultivation, and the cultivation temperature was 25°C ± 2 ℃, the light intensity is 4000Lux, and the photoperiod is 16 hours; it can be used for cryopreservation after 25 days of culture;

...

Embodiment 2

[0031] 1. Obtaining Xinjiang Snow Lotus Callus

[0032] Same as embodiment 1;

[0033] 2. The subculture of Xinjiang Saussurea chinensis callus

[0034] The Xinjiang snow lotus callus that step 1 induces is transferred to the MS medium containing α-naphthaleneacetic acid: 2.5mg / L, 6-benzylaminoadenine: 0.5mg / L for cultivation, and the sucrose concentration is adjusted in the medium To 4%, culture condition is identical with embodiment 1; Can be used for low atmospheric pressure pre-cultivation after cultivating 25 days;

[0035] 3. Low atmospheric pressure pre-cultivation of Xinjiang Snow Lotus callus

[0036] The Xinjiang Snow Lotus callus cultured for 25 days was transferred to a 40kP container and cultured for 4 days.

[0037] 4. Ultra-low temperature preservation of callus of Saussurea sauraceae in Xinjiang

[0038] Same as embodiment 1;

[0039] 5. Thawing and re-cultivation of ultra-low temperature cryopreserved Xinjiang Snow Lotus callus

[0040] The method is the...

Embodiment 3

[0042] 1. Obtaining Xinjiang Snow Lotus Callus

[0043] Same as embodiment 1;

[0044] 2. The subculture of Xinjiang Saussurea chinensis callus

[0045] The Xinjiang snow lotus callus that step 1 induces is transferred to the MS medium containing α-naphthaleneacetic acid: 2.5mg / L, 6-benzylaminoadenine: 0.5mg / L for cultivation, and the sucrose concentration is adjusted in the medium To 4%, culture condition is identical with embodiment 1; Can be used for low atmospheric pressure pre-cultivation after cultivating 25 days;

[0046] 3. Low atmospheric pressure pre-cultivation of Xinjiang Snow Lotus callus

[0047] The Xinjiang Snow Lotus callus cultured for 25 days was transferred to a 50kP container and cultured for 6 days.

[0048] 4. Ultra-low temperature preservation of callus of Saussurea sauraceae in Xinjiang

[0049] Same as embodiment 1;

[0050] 5. Thawing and re-cultivation of ultra-low temperature cryopreserved Xinjiang Snow Lotus callus

[0051]The method is the ...

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PUM

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Abstract

The present invention discloses a method for ultra low temperature storage of Saussurea involucrata Kar.et Kir callus, wherein the obtained Saussurea involucrata Kar.et Kir callus is subjected to subculture, low atmospheric pressure pre-culture, ultra low temperature storage, thawing and re-culture so as to significantly improve the revival rate of the callus after ultra low temperature storage. With the method, the technical basis is provided for germplasm conservation of Saussurea involucrata Kar.et Kir, and the method can be used for germplasm conservation of Saussurea involucrata Kar.et Kir.

Description

technical field [0001] The invention belongs to the field of biotechnology and relates to the preservation of plant callus, in particular to a method for ultra-low temperature preservation of Xinjiang Saussurea involucrata Kar.et Kir. Treatment can significantly improve the resurrection rate of callus tissue after cryopreservation, and provide a technical basis for Xinjiang Saussurea germplasm preservation. Background technique [0002] Saussurea sp. is a perennial herb belonging to the family Compositae (Comppositae) and thistle family (Trib. Cynareae Less.) in Xinjiang. It is a rare and precious plateau medicinal plant. Xinjiang Snow Lotus has the functions of dispelling cold and dehumidification, strengthening tendons and yang, anti-inflammatory and analgesic, and shrinking the uterus. It can treat cold pain in the lower abdomen of women, impotence, anti-aging and inhibit the proliferation of cancer cells. [0003] Xinjiang snow lotus is mainly distributed on alpine ice-...

Claims

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Application Information

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Patent Type & Authority Applications(China)
IPC IPC(8): A01H4/00A01N3/00
Inventor 郭斌尉亚辉何玮祁昊楠
Owner NORTHWEST UNIV(CN)
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