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Mixed formula preparation for controlling nilaparvata lugens stal

A mixed formula, brown planthopper technology, applied in the field of microorganisms, can solve problems such as increased mortality and hindered growth and reproduction of insects

Active Publication Date: 2014-08-06
CHINA JILIANG UNIV
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

The lack of symbiotic bacteria will directly cause the growth and reproduction of insects to be hindered, and the mortality rate will increase.

Method used

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  • Mixed formula preparation for controlling nilaparvata lugens stal
  • Mixed formula preparation for controlling nilaparvata lugens stal

Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0014] Embodiment 1: (the preparation method of amylase Streptomyces chromogenes D metabolite)

[0015] Follow these steps:

[0016] (1) Activation culture of strains: move the separated and preserved amylase chromogenic Streptomyces strain D to the slant of Gaoshi No. 1 in the test tube, and cultivate it until the slant of the test tube is covered with spores under the condition of 28°C±1°C;

[0017] (2) Fermentation culture: the fermentation medium contains 20g of soybean cake powder, 15g of corn flour, 10g of bran, and NH 4 NO 3 3g, (NH 4 ) 2 SO 4 5g, CaCO 3 5g, MgSO 4 1 g, sterilized, cooled to 40°C, inoculated under aseptic conditions a spore of a metalloplatinase amylase chromogenic Streptomyces D into the fermentation medium, and cultured for 5 days at 28°C ± 1°C;

[0018] (3) Extraction of fermented product: After the fermentation is completed, the fermented liquid is collected by centrifugation, added an equal volume of ethyl acetate for extraction, the lower l...

Embodiment 2

[0020] Embodiment 2: (preparation of amylase Streptomyces chromogenes D metabolite and imidacloprid mixed formula)

[0021] Imidacloprid water-dispersible granules (the active ingredient mass percentage is 70%) are produced by Bayer, and imidacloprid water-dispersible granules and amylase chromogenic Streptomyces D metabolites are dissolved with tap water respectively, mix, and be made into final concentration and be 0.2g / A mixed formula of L-amylase Streptomyces chromogenes D metabolite and 0.01g / L imidacloprid.

Embodiment 3

[0022] Embodiment 3: (Application of amylase Streptomyces chromogenicum D metabolite and imidacloprid mixed formula in the control of brown planthopper nymphs)

[0023] Follow these steps:

[0024] (1) TN1 paddy rice at the tillering and jointing stage, after removing part of the roots and base leaf sheaths, wash and dry naturally;

[0025] (2) Using clear water as a control, the final concentration of spraying is 0.2g / L amylase Streptomyces chromogenes D metabolite and 0.01g / L imidacloprid mixed agent, which requires uniform distribution of agent droplets, high coverage rate and appropriate dosage. It is advisable to wet the surface of the target object without loss, and let it dry naturally;

[0026] (3) Put the rice seedlings processed in step (2) into a large test tube injected with 20ml rice culture solution in advance, insert 3 rice seedlings into 3 instar brown planthopper nymphs in each tube, and use the rice seedlings after spraying clear water Treat as negative con...

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Abstract

The invention relates to the technical field of microorganisms, particularly to a Streptomyces diastatochromogenes D metabolite and imidacloprid mixed formula preparation. Streptomycesdiastatochromogenes is named Streptomycesdiastatochromogenes D. The strain is preserved in the CGMCC (China General Microbiological Culture Collection Center) of the China Committee for Culture Collection of Microorganisms on May25th, 2007 with the preservation register number of CGMCC No.2060. Metabolites capable of inhibiting nilaparvatalugensstal yeast symobiotic bacteria can be obtained through fermentation and extraction of the Streptomyces diastatochromogenes D. The most significant characteristic of the preparation is that the Streptomyces diastatochromogenes D metabolites and conventional insecticide imidacloprid are mixed in a certain proportion to prepare a new formula, after the mixed formula is used for controlling nilaparvata lugens stal, the usage amount of insecticides can be reduced obviously, the control effect on the nilaparvata lugens stal can be effectively improved, and the development of the drug resistance of the nilaparvata lugens stal can be delayed.

Description

technical field [0001] The invention relates to the technical field of microorganisms, in particular to a mixed formulation of amylase Streptomyces chromogenes D metabolites and imidacloprid. Background technique [0002] Brown planthopper (Nilaparvata lugens St 1) It is one of the important pests of rice, which mainly damages rice plants by sucking phloem juice and spreading rice viruses. When the pests occur, a large area of ​​rice plants will die and fall, resulting in reduced grain production, or even no harvest. In terms of production, chemical pesticides are the main means of controlling brown planthoppers. However, due to the large and frequent use of chemical insecticides, the brown planthopper has developed resistance to many chemical insecticides, resulting in a significant decline in the control effect. Therefore, it is imminent to study and apply new means to reduce the occurrence and damage of rice brown planthopper. [0003] The symbiosis between insects a...

Claims

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Application Information

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Patent Type & Authority Applications(China)
IPC IPC(8): A01N63/02A01P7/04A01N51/00
Inventor 李丹婷申屠旭萍俞晓平
Owner CHINA JILIANG UNIV
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