Looking for breakthrough ideas for innovation challenges? Try Patsnap Eureka!

SNP marker related to miniature pig type 2 diabetes and SNP marker detection method as well as application of SNP marker

A technology for type 2 diabetes and miniature pigs, applied in the field of molecular biology, can solve problems such as difficulty in obtaining spontaneous diabetic miniature pigs, and achieve the effect of wide application prospects

Active Publication Date: 2014-07-23
GUANGXI UNIV
View PDF2 Cites 0 Cited by
  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

However, it is very difficult to establish a type 2 diabetes model in large animals, and the probability of obtaining spontaneously diabetic miniature pigs is very rare. Therefore, it is urgent to screen and breed type 2 diabetes susceptible or resistant strains of miniature pigs

Method used

the structure of the environmentally friendly knitted fabric provided by the present invention; figure 2 Flow chart of the yarn wrapping machine for environmentally friendly knitted fabrics and storage devices; image 3 Is the parameter map of the yarn covering machine
View more

Image

Smart Image Click on the blue labels to locate them in the text.
Viewing Examples
Smart Image
  • SNP marker related to miniature pig type 2 diabetes and SNP marker detection method as well as application of SNP marker
  • SNP marker related to miniature pig type 2 diabetes and SNP marker detection method as well as application of SNP marker
  • SNP marker related to miniature pig type 2 diabetes and SNP marker detection method as well as application of SNP marker

Examples

Experimental program
Comparison scheme
Effect test

Embodiment

[0032] 1. Extraction of Minigroup Genomic DNA

[0033] Using the method of blood collection from the anterior vena cava, the blood of 46 experimental pigs in the preparatory example was collected, 3ml was taken from each case, put into a sodium citrate anticoagulant blood collection tube, and placed in a -20°C refrigerator for storage until use;

[0034] Genomic DNA of 46 pigs was extracted according to the operation method of the blood genomic DNA extraction kit of Beijing Tiangen Biotechnology Co., Ltd.

[0035] 2. Target fragment PCR amplification and sequencing

[0036]Use the extracted DNA as a template and carry out PCR amplification according to the designed primers: take 3 μL of DNA template, 2 μL of each primer shown in SEQ ID NO:2 and SEQ ID NO:3, 25 μL of PCRMix reagent, and 18 μL of double distilled water , set the PCR amplification system: pre-denaturation at 94°C for 5min; denaturation at 98°C for 10s, annealing at 61°C for 30s, extension at 72°C for 60s, 35 cyc...

the structure of the environmentally friendly knitted fabric provided by the present invention; figure 2 Flow chart of the yarn wrapping machine for environmentally friendly knitted fabrics and storage devices; image 3 Is the parameter map of the yarn covering machine
Login to View More

PUM

No PUM Login to View More

Abstract

The invention discloses an SNP marker related to miniature pig type 2 diabetes and an SNP marker detection method as well as an application of the SNP marker. The SNP marker is G / A polymorphism located at a part 325bp of an exon No.1 of a Leptin gene, and the nucleotide sequence of the Leptin gene with the SNP marker is as shown in SEQ ID No.1. The SNP marker detection method comprises the following steps: extracting genome DNA of a miniature pig, which is used as a template, using a primer, the nucleotide sequences of which are as shown in SEQ ID No.2 and SEQ ID No.3, for carrying out PCR amplification, sequencing a PCR amplification product, analyzing a sequencing result, and identifying the G / A polymorphism at the part 325bp of the exon No.1. The SNP marker provided by the invention is susceptibly related to the miniature pig type 2 diabetes, and therefore, miniature pig strains susceptible to or resisting the type 2 diabetes are screened by identifying the SNP marker.

Description

technical field [0001] The invention belongs to the technical field of molecular biology, and relates to a SNP marker related to type 2 diabetes in minipigs, a detection method and application thereof. Background technique [0002] Diabetes mellitus (DM) is a chronic endocrine and metabolic disease that seriously threatens human health, and its main symbol is hyperglycemia. In recent years, the global prevalence of diabetes has shown a rapid upward trend. Statistics from the World Health Organization in 2011 show that the number of diabetic patients in the world has reached 366 million, and it is estimated that the global incidence will reach 439 million by 2030. The prevalence of diabetes among residents aged 18 and over in my country is 9.7%, and the prevalence of elderly people over 60 years old is as high as 19.6%. There are about 97 million adult diabetic patients in the country. Diabetes has become a major threat to the health of our population, especially the health o...

Claims

the structure of the environmentally friendly knitted fabric provided by the present invention; figure 2 Flow chart of the yarn wrapping machine for environmentally friendly knitted fabrics and storage devices; image 3 Is the parameter map of the yarn covering machine
Login to View More

Application Information

Patent Timeline
no application Login to View More
IPC IPC(8): C12N15/11C12Q1/68
Inventor 郭亚芬兰干球蒋钦杨杨辉
Owner GUANGXI UNIV
Who we serve
  • R&D Engineer
  • R&D Manager
  • IP Professional
Why Patsnap Eureka
  • Industry Leading Data Capabilities
  • Powerful AI technology
  • Patent DNA Extraction
Social media
Patsnap Eureka Blog
Learn More
PatSnap group products

Browse by: Latest US Patents, China's latest patents, Technical Efficacy Thesaurus, Application Domain, Technology Topic, Popular Technical Reports.

© 2024 PatSnap. All rights reserved.Legal|Privacy policy|Modern Slavery Act Transparency Statement|Sitemap|About US| Contact US: help@patsnap.com