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Preparation of Ganoderma lucidum spore powder fermented by Bacillus subtilis bs01 and its application in improving sleep

A technology of Bacillus subtilis and Ganoderma lucidum spore powder, applied to medical preparations containing active ingredients, bacteria, plant/algae/fungus/moss components, etc.

Inactive Publication Date: 2017-12-22
SICHUAN AGRI UNIV
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

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Problems solved by technology

The present inventor has to Bacillus subtilis ( Bacillus subtilis ) BS01 CCTCCNO:M2012485 and unfermented Ganoderma lucidum spore powder in improving sleep (publication number is CN103361290A), but the preparation of Bacillus subtilis BS01 fermented Ganoderma lucidum spore powder and whether it can improve sleep quality effect, needs further study

Method used

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  • Preparation of Ganoderma lucidum spore powder fermented by Bacillus subtilis bs01 and its application in improving sleep

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Experimental program
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Effect test

Embodiment 1

[0013] Example 1 Condition optimization for fermentation of Ganoderma lucidum spore powder by "Bacillus subtilis CCTCC 2012485" of the present invention

[0014] Studies have reported that concentrations of 10 5 CFU / g of Bacillus subtilis can effectively improve sleep in mice. In order to ensure the number of live bacteria in the "Bacillus subtilis CCTCC 2012485" fermented ganoderma spore powder of the present invention, the present invention optimizes the concentration of the "Bacillus subtilis CCTCC 2012485" fermented ganoderma spore powder.

[0015] Ganoderma lucidum spore powder was prepared into a suspension with a concentration of 6.6 mg / mL by using distilled water, normal saline, 10% LB medium (Luria-Bertani, LB) and LB medium respectively. Bacillus subtilis BS01 was inoculated into 50ml of the above suspension at 1% inoculation amount, and fermented at 37°C for 12h, 24h, 48h, and 72h. Viable bacteria were counted after the fermentation broth was heat-treated (80°C) f...

Embodiment 2

[0023] Example 2 Effect of "Bacillus subtilis CCTCC 2012485" fermented Ganoderma lucidum spore powder of the present invention on the sleep of mice

[0024] The fermentation broth of the present invention is prepared by inoculating Bacillus subtilis BS01 into a suspension of ganoderma lucidum spore powder with a concentration of 6.6 mg / mL prepared in physiological saline and fermenting for 24 hours. Viable count was 2.6×10 6 CFU / mL.

[0025] According to the relevant provisions of the national health food management regulations, 36 male clean-grade Kunming mice (body weight 20.0±0.5g) were selected and randomly divided into three groups, 12 in each group. The experimental period was 30 days, and the mice were fed with basal diet and gavaged at the same time. According to routine feeding and management, free access to water and food.

[0026] Experimental detection data:

[0027] The influence of the "Bacillus subtilis CCTCC 2012485" fermented Ganoderma lucidum spore powde...

Embodiment 3

[0041] Example 3 Formula and preparation method of microecological preparation of "Bacillus subtilis CCTCC 2012485" fermented Ganoderma lucidum spore powder of the present invention

[0042] Probiotic formulation:

[0043]

[0044] Preparation method: The fermented product of Bacillus subtilis BS01 and Ganoderma lucidum spore powder is light yellow powder, inoculated with Bacillus subtilis BS01 to 6.6 mg / mL Ganoderma lucidum spore powder suspension for fermentation, the suspension is prepared from normal saline, after 24 hours of fermentation Add 5% skimmed milk powder, and use freeze-drying process at -45°C to obtain freeze-dried powder. Freeze-dried powder and other raw materials are mixed together according to the prescription ratio, then sterilized under high temperature and high pressure at 121°C for 15 minutes, made into soft material with 75% alcohol, and then made into wet granules with a 40-mesh sieve, dried at 50°C until the moisture content is 5% Next, compress ...

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Abstract

The invention discloses the application of ganoderma lucidum spore powder fermented by Bacillus subtilis (Bacillus subtilis) BS01 in improving sleep. The fermentation of Ganoderma lucidum spore powder by Bacillus subtilis BS01 significantly reduced the sleep latency and serum MDA content of mice. The sleep time, subthreshold hypnotic sleep rate, T‑AOC activity, SOD activity, CAT activity and GSH‑Px activity of the mice were increased by 41.44%, 66.67%, 24.66%, 19.49%, 55.68% and 31.27%, respectively. The Ganoderma lucidum spore powder fermented by Bacillus subtilis BS01 can effectively improve the sleep quality of mice, increase the antioxidant capacity of serum, and can be used to prepare microecological preparations for improving sleep.

Description

technical field [0001] The invention relates to the application of ganoderma lucidum spore powder fermented by Bacillus subtilis BS01 in improving sleep. Background technique [0002] In recent years, social problems caused by lack of sleep have become increasingly prominent, and sleep problems have become a prominent problem that threatens human health. In our country, with the improvement of national living standards, people began to pursue a more comfortable way of life. Sleep quality and sleep environment have become important indicators to measure people's quality of life. The 2013 China Sleep Index shows that 55.5% of Chinese people believe that work pressure has a great impact on sleep, 55.3% of Chinese people use various sleep aids, and 14 out of 100 Chinese people have not fallen asleep beyond 12 o'clock in the morning. Therefore, the development of microecological agents that can improve sleep has broad social significance. [0003] However, most of the current ...

Claims

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Application Information

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Patent Type & Authority Patents(China)
IPC IPC(8): C12N1/20A61K36/074A61P25/20A61K35/742
Inventor 倪学勤曾东罗慧周梦佳潘康成景波李涛王剑马源晨祝辉
Owner SICHUAN AGRI UNIV
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