Looking for breakthrough ideas for innovation challenges? Try Patsnap Eureka!

A kind of preparation method and application of NK cytokine mixture

A technology of NK cells and cytokines, which is applied in the field of preparation of NK cytokine mixtures, and can solve the problems of cumbersome cell culture steps

Inactive Publication Date: 2016-03-30
湖北华赛生物医药技术有限公司
View PDF3 Cites 1 Cited by
  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

[0008] Traditional NK cell culture methods need to use PHA and / or tumor cell lines (such as K562, HFWT, and K562-MB15-41BBL) as stimulating factors and trophoblasts to cultivate NK cells, which has cumbersome cell culture steps, using PHA and tumor cells Strains also pose safety concerns

Method used

the structure of the environmentally friendly knitted fabric provided by the present invention; figure 2 Flow chart of the yarn wrapping machine for environmentally friendly knitted fabrics and storage devices; image 3 Is the parameter map of the yarn covering machine
View more

Image

Smart Image Click on the blue labels to locate them in the text.
Viewing Examples
Smart Image
  • A kind of preparation method and application of NK cytokine mixture

Examples

Experimental program
Comparison scheme
Effect test

Embodiment Construction

[0019] 1. In vitro culture, expansion and activation of NK cells

[0020] 1) Under sterile conditions, draw 20ml of peripheral blood from the patient's vein into a 50ml centrifuge tube, and add 300IU heparin sodium anticoagulant;

[0021] 2) Add physiological saline or PBS to dilute at 1:1;

[0022] 3) Add 5ml of lymphocyte separation medium to an empty 15ml centrifuge tube, take 10ml of diluted blood and slowly add it to a 15ml centrifuge tube, centrifuge at 1500rpm for 20min, take buffy coat cells and wash with normal saline, centrifuge at 1200rpm for 10min, then resuspend in PBS , repeat once, discard the supernatant;

[0023] 4) Resuspend the above cells with NK cell medium, and add anti-CD3 antibody (OrthocloneOKT-3; OrthoBiotech, Raritan, NJ), and adjust the final concentration of antibody to 10ng / ml;

[0024] NK cell culture medium Manufacturer Serum-free stem cell growth medium SCGM CellGenix, Germany 5% human serum Biowhittaker-Cambrex, USA ...

the structure of the environmentally friendly knitted fabric provided by the present invention; figure 2 Flow chart of the yarn wrapping machine for environmentally friendly knitted fabrics and storage devices; image 3 Is the parameter map of the yarn covering machine
Login to View More

PUM

No PUM Login to View More

Abstract

The invention belongs to the field of tumor biotherapy, and relates to a preparation method and an application of a NK cells-cytokines mixture prepared by using an activation method for NK cells. NK cells obtained according to the invention are tumor-killing cells which are cultured in vitro, activated and amplified and has a cytotoxic effect, and can directly kill tumor cells and enhance immunity after being fed back to the bodies of patients; NK cell culture supernatant prepared according to the invention and freeze-dried powder preparations prepared by freeze-drying and concentrating the NK cell culture supernatant contain a series of natural cytokine mixtures with bioactivity, and the mixture of natural cytokines has good antitumor activity in anti-tumor treatment.

Description

technical field [0001] The invention belongs to the field of tumor biotherapy, and relates to a preparation method and application of NK cytokine mixture. Background technique [0002] Natural killer cells (Natural killer cells, NK) are one of the first innate immune defense members in the human body, and they are also the strongest and most effective immune lymphocytes against cancer cells or viruses. Compared with T cells (Cytotoxic T cells, CTL) or dendritic cells (Dendritic cells, DC), NK cells have a broad spectrum, are not limited by histocompatibility antigens, and can directly launch an attack on cancer cells without the initiation of sensitization. attack, hence the name natural killer cells. NK cells play the role of immune surveillance on a variety of infectious microorganisms and tumor cells, and have anti-tumor, anti-infection and immune regulation functions. Their target cells mainly include tumor cells, virus-infected cells, and some self-tissue cells (such a...

Claims

the structure of the environmentally friendly knitted fabric provided by the present invention; figure 2 Flow chart of the yarn wrapping machine for environmentally friendly knitted fabrics and storage devices; image 3 Is the parameter map of the yarn covering machine
Login to View More

Application Information

Patent Timeline
no application Login to View More
Patent Type & Authority Patents(China)
IPC IPC(8): C12N5/0783A61K35/26A61P35/00A61K35/17
Inventor 吴皖王雄伟汤威邢文彦罗琳
Owner 湖北华赛生物医药技术有限公司
Who we serve
  • R&D Engineer
  • R&D Manager
  • IP Professional
Why Patsnap Eureka
  • Industry Leading Data Capabilities
  • Powerful AI technology
  • Patent DNA Extraction
Social media
Patsnap Eureka Blog
Learn More
PatSnap group products

Browse by: Latest US Patents, China's latest patents, Technical Efficacy Thesaurus, Application Domain, Technology Topic, Popular Technical Reports.

© 2024 PatSnap. All rights reserved.Legal|Privacy policy|Modern Slavery Act Transparency Statement|Sitemap|About US| Contact US: help@patsnap.com