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Anti-liver cancer stem cell monoclonal antibody and its application

A monoclonal antibody and stem cell technology, applied in the direction of antibodies, anti-tumor drugs, anti-animal/human immunoglobulin, etc., can solve the problems of clinical application impact, unsatisfactory specificity of anti-liver cancer monoclonal antibody, etc., and achieve The effect of strong proliferative ability

Inactive Publication Date: 2016-01-20
THE FIRST AFFILIATED HOSPITAL OF THIRD MILITARY MEDICAL UNIVERSITY OF PLA
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  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

Since the advent of monoclonal antibody technology, people have tried to improve the early diagnosis rate and treatment effect of liver cancer through the application of anti-liver cancer monoclonal antibodies, but the specificity of anti-liver cancer monoclonal antibodies obtained so far is not satisfactory, due to tumor heterogeneity The existence of , and its clinical application is affected to a certain extent

Method used

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  • Anti-liver cancer stem cell monoclonal antibody and its application
  • Anti-liver cancer stem cell monoclonal antibody and its application
  • Anti-liver cancer stem cell monoclonal antibody and its application

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Experimental program
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Embodiment Construction

[0020] The preferred embodiments of the present invention will be described in detail below with reference to the accompanying drawings.

[0021] 1. Mouse anti-human liver cancer stem cell PLC-Nanog POS Preparation of monoclonal antibodies

[0022] 1. Mouse anti-human liver cancer stem cell PLC-Nanog POS Obtaining hybridoma cell lines

[0023] The cultured PLC-Nanog POS Nanog neg Cells (i.e. liver cancer cells PLC with Nanog as promoter to regulate GFP expression) were sorted by flow cytometry to select PLC-Nanog POS Cells (i.e. Nanog-positive PLC cells) were used as immunogens; healthy BALB / c mice aged 4-6 weeks with a body weight of 14-18 g were injected intraperitoneally with 1×10 6 PLC-Nanog POS The cells were immunized for the first time, and then boosted immunized twice in the same way every 2 weeks. On the 10th day after the last booster immunization, the tail blood of the mice was collected, the serum was separated, and the antibody titer was detected by the ind...

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Abstract

The invention discloses an anti-hepatoma stem cell monoclonal antibody which is secreted by a mouse hybridoma cell 4P-120 with the collection number of CGMCC No.8800. Proven by experiments, the monoclonal antibody can be specifically combined with hepatoma cells and is capable of specifically recognizing hepatoma tissues, antigens recognized by the monoclonal antibody are highly expressed in cancer tissues of human hepatoma, but are not expressed or slightly expressed in para-carcinoma tissues, and positive-to-negative differentiation and negative-to-positive defifferentiation exist in a cell multiplication process. Compared with cells with low antigen expression, cells with high antigen expression have higher proliferation capability, clone formation capability, balling capability, scratch transfer capability and invasion transfer capability; and the invention provides a functional monoclonal antibody with an application prospect for treating hepatoma with targeted tumor stem cells, and the monoclonal antibody can be used for preparing medicines for treating hepatoma.

Description

technical field [0001] The invention belongs to the field of biotechnology and relates to a monoclonal antibody. Background technique [0002] Liver cancer is one of the most common malignant tumors in my country, and its mortality rate ranks third in malignant tumors. The 5-year survival rate of liver cancer patients is only 14% to 30%. Primary liver cancer has the characteristics of insidious onset and high mortality rate. Early diagnosis and prevention of recurrence are of great significance to prognosis. Since the advent of monoclonal antibody technology, people have tried to improve the early diagnosis rate and treatment effect of liver cancer through the application of anti-liver cancer monoclonal antibodies, but the specificity of anti-liver cancer monoclonal antibodies obtained so far is not satisfactory, due to tumor heterogeneity The existence of it has affected its clinical application to a certain extent. Therefore, it is necessary to find better anti-hepatic c...

Claims

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Application Information

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Patent Type & Authority Patents(China)
IPC IPC(8): C07K16/18A61K39/395A61P35/00
Inventor 钱程洪娟赵文旭沈俊杰单娟娟
Owner THE FIRST AFFILIATED HOSPITAL OF THIRD MILITARY MEDICAL UNIVERSITY OF PLA
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