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Method of adopting fluorescence quenching system to potential fingerprint display

A fluorescence quenching and systematic technology, applied in the field of human identification, etc., can solve problems that have not been reported

Inactive Publication Date: 2014-07-16
SOUTH CENTRAL UNIVERSITY FOR NATIONALITIES
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

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Problems solved by technology

[0005] After testing, our idea has been verified, but this method has not been reported yet

Method used

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  • Method of adopting fluorescence quenching system to potential fingerprint display
  • Method of adopting fluorescence quenching system to potential fingerprint display
  • Method of adopting fluorescence quenching system to potential fingerprint display

Examples

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Embodiment 1

[0028] Example 1 The synthesis of nano-gold can be referred to: Lu Bingfeng, Li Guoping, Luo Yunjun. Aqueous phase synthesis and fluorescence properties of gold nanoparticles [J]. Chemical Journal of Chinese Universities, 2010, 31(8): 1502-1505; or: Zheng Haixia, Huang Boneng, Hu Junman, Gong Gong. Preparation method and performance characterization of gold nanoparticles with specific size [J]. Journal of Peking University (Natural Science Edition), 2011,47(5):777~782.

[0029] Specifically, a nano-gold-fluorescein fluorescence quenching system is used in a method for displaying latent fingerprints, the steps of which are as follows:

[0030] 1. Preparation of nano-gold: Weigh 0.60g of chloroauric acid, dissolve in distilled water, transfer to a 100mL volumetric flask, and distill to volume to obtain a yellow chloroauric acid solution; weigh 3.40g of trisodium citrate, dissolve in distilled water, and transfer to 100mL Volumetric flask, distilled water to volume, prepared tris...

Embodiment 2

[0036] A kind of nano gold-fluorescein fluorescence quenching system is used for the method for latent fingerprint display, and its steps are as follows:

[0037] 1. Nano-gold preparation: Weigh 0.60g of chloroauric acid, dissolve in distilled water, transfer to a 100mL volumetric flask, and distill to volume to obtain a yellow chloroauric acid solution; weigh 3.40g of trisodium citrate, dissolve in distilled water, and transfer to 100mL Volumetric flask, distilled water to volume, prepared trisodium citrate solution; weighed 0.10g polyvinylpyrrolidone, dissolved in distilled water and transferred to 100mL volumetric flask, distilled water to volume, prepared protective agent PVP solution. Take 5mL of trisodium citrate solution and 25mL of PVP solution in a round bottom flask, heat in a water bath for 10min, add 50mL of chloroauric acid solution and heat for another 10min; stop heating, shake the flask by hand for 5min (for stirring), cool to room temperature with tap water, P...

Embodiment 3

[0041] The preparation of water-soluble CdTe quantum dots-silver ion fluorescence quenching system can be referred to: Xu Zhixiang, Li Haibing, Chang Xueling, Yang Xiangliang, etc. Research on new silver ion nanofluorescent probes[J]. Journal of Analytical Science, 2007, 23( 4): 453-456.

[0042] Specifically, a water-soluble CdTe quantum dot-silver ion fluorescence quenching system is used for a method for latent fingerprint display, and the steps are as follows:

[0043] 1. Preparation of water-soluble CdTe quantum dots: under constant stirring, add thioglycolic acid (TGA) to a 2.0×10 -4 mol / L cadmium nitrate solution, add freshly prepared oxygen-free NaHTe solution, wherein the molar ratio of each substance is Cd 2+ :Te-:TGA=1:0.5:3. The reaction solution is heated to reflux to obtain a CdTe nanoparticle solution with a relatively uniform size. The theoretical concentration of the water-soluble CdTe quantum dots prepared in this experiment is 1.0×10 -4 mol / L.

[0044] ...

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Abstract

The invention relates to the technical field of trace amount detection, and discloses a method of adopting a fluorescence quenching system to potential fingerprint display. The method is characterized in that potential fingerprint is displayed by utilizing 'turn-on' effect of the fluorescence quenching system to transmit fluorescence once again and includes: firstly, preparing the fluorescence quenching system, wherein the quenching state can be destroyed by fingerprint components; then spraying and painting the fluorescence on various objects with fingerprints, wherein the fingerprint components of chloride ion, amino acid or glucose and the like destroy the quenching state of the fluorescence quenching system to enable the fluorescence to appear to display the fingerprints; taking photos directly through a filter under special light or under natural light. The method is simple and rapid; when fingerprint details are not clear enough, common components like DFO (1.8-diazo-flurene-9-one), IND (indanedione), ninhydrin, silver nitrate and the like are used for continuously displaying the fingerprints, and the method can be further used for 'marking' or 'tracing' the fingerprints.

Description

technical field [0001] The invention relates to the technical field of trace detection, in particular to a method for displaying latent fingerprints using a fluorescence quenching system. Fingerprint method. Background technique [0002] Fingerprints have the characteristics of "differing from person to person and remain unchanged for life". Based on this, the person involved in the case can be locked. It was once the "king of evidence". At present, due to the development and popularization of DNA technology, fingerprints and DNA are listed as the "king of evidence". On May 30, 2012, the newly revised "Resident Identity Card Law" clearly stipulated that the registration items of resident identity cards include fingerprint information. Therefore, the research on latent fingerprint visualization technology still has important application value. [0003] At room temperature, the methods that can display latent fingerprints within a few minutes mainly include powder method, 5...

Claims

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Application Information

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IPC IPC(8): A61B5/117
Inventor 王利华胡军成陈欢黄正喜
Owner SOUTH CENTRAL UNIVERSITY FOR NATIONALITIES
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