Method for preparing fermented coffee with decreased caffeine using plant loctobacillus
A plant lactobacillus, coffee technology, applied in the direction of coffee spice, green coffee processing, coffee reduction of alkali content, etc., can solve the problems of mixing taste and flavor, green coffee beans losing taste and flavor, etc.
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Embodiment 1
[0042] cultivating seeds
[0043] Fermenting lactic acid bacteria JS (Korean Patent No. 0435168, KCCM10499), which is a plant lactic acid bacterium, was mixed with 1×10 5 -1×10 6 The amount of cfu / ml was injected into the sterilized (121°C, 15 minutes) MRS medium, and shake cultured at 45°C for 15 hours. The cultured Lactobacillus plantarum was concentrated by centrifugation and freeze-dried. Then, according to the general method, sugar (such as glucose, lactose, etc.) is added to the dried Lactobacillus plantarum to prepare a high-concentration plantar Lactobacillus powder. In this case, the number of Lactobacillus plantarum was 2.5×10 11 cfu / g. Since Lactobacillus plantarum is used as Lactobacillus from crops, in addition to Lactobacillus fermentum JS (KCCM10499), Lactobacillus brevis, Lactobacillus casei, Lactobacillus plantarum, Leuconostoc enterococci, Pediococcus pentosaceae, Lactobacillus sake can be used , Leuconostoc lactis, and Leuconostoc citricophilus plant o...
Embodiment 2
[0044] Fermentation of green coffee beans (liquid fermentation of steeping solution)
[0045] Fresh Ethiopian Harrar (Harrar) G4Ambassa was used as green coffee beans, and the liquid fermentation of the steeping solution was carried out at a temperature of 42°C for 4 hours. Twice the amount added), controlled at 1×10 8 A range of cfu / ml was prepared.
Embodiment 2-1
[0047] Fermentation was carried out in the same manner as in Example 2 using NY2 17 / 18FC Cerrado Fazenda Bau estate boutique in Santos, Brazil.
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