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Bdellovibrio particulate agent

A leech vibrio and granule technology, applied in the field of microbial preparations, can solve the problems of large damage to microbial cells, high price of sodium alginate, and high cost of leech vibrio granules, and achieve stable chemical properties, long storage time, and mechanical strength. high effect

Inactive Publication Date: 2014-07-09
QINGDAO JINLIANXIN BUSINESS & TRADE
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

However, the formation conditions of the polymer network are relatively severe, the damage to the microbial cells is relatively large, and the diversity and controllability of the formation are not good.
Zhang Xiaoneng et al. studied the optimization of the preparation conditions of Bdellovibrio granules by sodium alginate embedding method in "Optimization of Preparation Conditions for Bdellovibrio Phage Granules". Vibrio granules are expensive and not suitable for large-scale industrial production

Method used

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Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0009] A kind of Bdellovibrio granule, it is the agar that is 5% by peanut oil, mass concentration and Bdellovibrio content is 10 11 The CFU / mL bacterial solution was mixed according to the volume ratio of 3: 50: 100, then added with 2% sodium carboxymethyl cellulose and mixed evenly, and the mixed solution was dropped into the CaCl2 with a mass fraction of 10%. solidified in the solution for 12 hours, and then freeze-dried the Bdellovibrio solidified particles at -20°C for 24 hours to obtain granules. Its specific preparation method is:

[0010] (1) Preparation of Bdellovibrio bacteria liquid:

[0011] Inoculate 100 mL of Bdellovibrio strains into 1 / 10 NB medium, add 100 L of Escherichia coli suspension with a concentration of 1.0×106 CFU / mL, and shake the culture at 35°C and 100 r / min for 48 hours. Detect the total number of colonies, and the concentration of Bdellovibrio bacteria liquid is 1.0×10 11 CFU / mL for use.

[0012] (2) Preparation of Bdellovibrio embedding part...

Embodiment 2

[0015] A kind of Bdellovibrio granule, its concrete preparation method is:

[0016] (1) Preparation of Bdellovibrio bacteria liquid:

[0017] Inoculate 100 mL of Bdellovibrio strains into 1 / 10 NB medium, add 100 L of Escherichia coli suspension with a concentration of 1.0×106 CFU / mL, and shake the culture at 35°C and 100 r / min for 48 hours. Detect the total number of colonies, and the concentration of Bdellovibrio bacteria liquid is 1.0×10 10 CFU / mL for use.

[0018] (2) Preparation of Bdellovibrio embedding particles:

[0019] Peanut oil, agar with mass concentration of 10% and Bdellovibrio content as 10% 10 The CFU / mL bacterial solution was put into a stirring container according to the volume ratio of 3: 50: 100, and then sodium carboxymethyl cellulose with a mass concentration of 2% was added, and stirred at a constant temperature of 25°C for 30 minutes to make it evenly mixed. The mixed solution was dropped into a CaCl2 solution with a mass fraction of 10% to solidify...

Embodiment 3

[0021] Detection of live bacteria count in solidified granules

[0022] The Bdellovibrio granules of the present invention can be stored at normal temperature, and the Bdellovibrio granules with different storage times at normal temperature are taken to detect the number of viable bacteria, and the Bdellovibrio granules prepared in Example 1 are used as experimental objects.

[0023] Take 0.1 g of the Bdellovibrio granules prepared in Example 1, add 10 mL of 0.055 mol / L sodium citrate solution, after the granules are completely disintegrated, mix well, and after gradient dilution, use tap water double-layer agar plate method to detect The live bacteria content of Bdellovibrio in the granules, and finally calculate the number of live bacteria of Bdellovibrio per gram of granules. After testing, the storage time is within 6 months, and the concentration of live bacteria in the prepared Bdellovibrio granules is from 1.0×10 11 CFU / mL dropped steadily to 5×10 10 CFU / mL, the decre...

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Abstract

A bdellovibrio particulate agent is characterized by being prepared by: mixing plant oil, agar with a mass concentration of 5%-10%, and a bacteria liquid with a bdellovibrio content of 10<10>-10<11> CFU / mL according to a volume ratio of 3:50:100, then adding sodium carboxymethylcellulose with a mass concentration of 2% and mixing uniformly, dropwise adding the mixed solution into a CaCl2 solution with a mass percentage of 10%, solidifying for 12 h, and then performing freeze drying at -20 DEG C for 24 h on bdellovibrio solidified particles to obtain the particulate agent. The bdellovibrio particulate agent is high in mechanical strength, the bdellovibrio activity is well stored, and the bdellovibrio particulate agent is stable in chemical performances, low in cost and long in storage time, and is applicable to multiple industries such as aquatic product culture, livestock and poultry culture and the like.

Description

technical field [0001] The invention relates to a microbial preparation, in particular to a bacteriophage Bdellovibrio granule. Background technique [0002] Bdellovibrio bacteria (Bdellovibrio bacteriovorus) (hereinafter referred to as Bdellovibrio) is a parasitic bacterium that specializes in preying on bacteria. Some studies have pointed out that Bdellovibrio bacteria can purify the water quality of aquaculture water and control or reduce aquatic animal diseases. The effect on occurrence and prevalence is remarkable. Adding Bdellovibrio to the breeding process can prevent and control diseases, improve survival rate, increase body weight and length, and purify the breeding ecological environment. Chen Liyun et al found that adding Bdellovibrio can reduce the incidence of turbot redmouth disease from 10.17% to 0.93%, indicating that Bdellovibrio can effectively prevent the occurrence of redmouth disease. In addition, in the treatment of redmouth disease , Red mouth disease...

Claims

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Application Information

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IPC IPC(8): A01N25/12A01N63/00A01P1/00
Inventor 黄艳
Owner QINGDAO JINLIANXIN BUSINESS & TRADE
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