Detection method for ethyl acetate extract of paederia scandens
A technology of ethyl acetate extraction and detection method, which is applied in measurement devices, instruments, scientific instruments and other directions, and can solve problems such as applications that have not yet been seen
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[0034] 1. Experimental reagents
[0035] Methanol is chromatographically pure, from Fisher Corporation of the United States;
[0036] Ultrapure water was prepared by laboratory ELGA PURELAB Classic-UVF pure water machine, which was purchased from the UK;
[0037] All other reagents were commercially available domestic analytical grade.
[0038] 2. Experimental samples
[0039] Patent sample: Ethyl acetate extract of Caulis chinensis prepared in Example 1 of CN201210209139.8. The specific preparation method is as follows: the medicinal material sample is crushed into coarse powder, and 500 grams of coarse powder is taken, soaked in petroleum ether for 1 day, and then percolated Extract until the color of the extract is light (or 5 times the amount of medicinal materials), recover petroleum ether to obtain petroleum ether extract; evaporate the residue to remove petroleum ether, moisten it with 80% ethanol, and then use 3 times the amount of 80% ethanol to reflux and extract t...
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