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Method for separating and purifying curdlan by use of membrane process

A technology of separation, purification and process, applied in the field of industrialized production of curdlan gum, which can solve the problems of low gel strength, high ion concentration, low gel transparency, etc.

Active Publication Date: 2014-07-02
山东萃源亿康生物科技有限公司
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

[0005] In view of the above-mentioned prior art, in order to solve the problems of low purity, high ion concentration, low gel strength, and low transparency of curdlan products at present, the present invention provides a method for purifying curdlan by using a membrane separation process. glue method

Method used

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Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0019] Example 1 Separation and Purification of Curdlan Gum Using Membrane Technology

[0020] Proceed as follows:

[0021] (1) Dilute alkali solution: take curdlan fermentation broth, add it to sodium hydroxide solution, stir continuously to dissolve it completely, and control the concentration of curdlan gum in the solution to 0.5% (w / v, g / ml), [OH - ] concentration is 0.5mol / L;

[0022] (2) Microfiltration membrane filtration for impurity removal: the dissolving liquid adopts a ceramic microfiltration membrane with a pore size of 50nm to remove microbial cells and insoluble impurities; control the temperature of the dissolving liquid during the operation process below 30°C, and collect the filtrate; When the concentration reaches 1% (w / v, g / ml), add sodium hydroxide solution to make [OH - ] Concentration is 0.5mol / L, continue to concentrate, until 90% curdlan in the solution is taken out by filtrate, finish filtration operation;

[0023](3) Neutralization and precipita...

Embodiment 2

[0026] Example 2 Separation and Purification of Curdlan by Membrane Technology

[0027] Proceed as follows:

[0028] (1) Dilute alkali solution: add curdlan gum crude product to the sodium hydroxide solution, stir continuously to dissolve it completely, the concentration of curdlan gum in the solution is 0.4% (w / v, g / ml), [OH - ] concentration is 0.3mol / L;

[0029] (2) Microfiltration membrane filtration to remove impurities: the solution is filtered through a ceramic microfiltration membrane with a pore size of 100nm to remove microbial cells and insoluble impurities; control the operation process to keep the temperature of the gum base solution below 30°C, and collect the filtrate; When the retentate solids concentration reaches 1% (w / v), add sodium hydroxide solution to make [OH - ] Concentration is 0.5mol / L, continue to concentrate, until 90% curdlan in the solution is taken out by filtrate, finish filtration operation;

[0030] (3) Neutralization and precipitation: co...

Embodiment 3

[0033] Example 3 Separation and Purification of Curdlan by Membrane Technology

[0034] Proceed as follows:

[0035] (1) Dilute alkali solution: add curdlan gum fermentation liquid to potassium hydroxide solution, stir continuously to dissolve it completely, and finally curdlan gum concentration is 0.4% (w / v, g / ml), [OH - ] concentration is 0.3mol / L;

[0036] (2) Microfiltration membrane filtration to remove impurities: the solution is filtered through a ceramic microfiltration membrane with a pore size of 100nm to remove microbial cells and insoluble impurities; the temperature of the gum solution in the control operation project can be kept below 30°C, and the filtrate is discharged ; Whenever the retentate solids concentration reaches 1% (w / v), potassium hydroxide solution is supplemented so that the [OH-] concentration is 0.5mol / L, and the concentration is continued until 90% curdlan in the solution is filtered The liquid is taken out to end the filtration operation;

...

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Abstract

The invention discloses a method for purifying curdlan by use of a membrane separation process. The method comprises the steps of dissolving curdlan fermentation broth or a crude curdlan product in an alkaline solution; by means of the principle that curdlan molecules completely stretched in an aqueous solution can pass through a microfiltration membrane with an aperture higher than 10 nm and microbial bacteria and insoluble impurities can not pass through the microfiltration membrane and the advantage that a ceramic microfiltration membrane is resistant to acid and alkali, separating and removing the microbial bacteria and insoluble impurities in curdlan aqueous alkali by filtering through the ceramic microfiltration membrane, collecting to obtain clear curdlan aqueous alkali with relatively low concentration, adding acid in the clear curdlan aqueous alkali for neutralization to separate out the curdlan in the form of neutralization gel, filtering and concentrating by using an ultrafiltration membrane to obtain the curdlan neutralization gel, adding water to wash and desalt, and finally dewatering and drying through an organic solvent to obtain a high-quality curdlan product. The dry purity of the product exceeds 95%, the gel strength is increased to 1000-1200 g / cm<2>, and the performance is stable.

Description

technical field [0001] The invention relates to a method for refining curdlan gum, in particular to a method for purifying curdlan gum by using membrane separation technology, and belongs to the technical field of curdlan gum industrial production. Background technique [0002] Curdlan gum (Curdlan) is a microbial exopolysaccharide, which has the unique property of forming a gel under heating conditions, also known as thermogel or thermocoagulation polysaccharide. Curdlan gum molecules are unbranched homogeneous polysaccharide polymers connected by β-1,3-D-glucosidic bonds, composed of about 300 to 500 glucose residues, with an average relative molecular weight of about 74000D (Daltons) . Curdlan gum is safe and non-toxic to the human body, insoluble in water but soluble in alkaline solution to form a viscous solution. Curdlan gum is widely used in the food industry as a food additive, which can improve the water holding capacity, viscoelasticity and stability of the produ...

Claims

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Application Information

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Patent Type & Authority Applications(China)
IPC IPC(8): C08B37/00
Inventor 董学前吉武科武琳张永刚刘建军
Owner 山东萃源亿康生物科技有限公司
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