Multi-gene detection kit for selection of chemotherapy regimens

Abstract

The invention relates to a multi-gene detection kit for the selection of chemotherapy regimens. The multi-gene detection kit comprises a mixture of RT (reverse transcriptase primer) primers and a mixture of PCR (Polymerase Chain Reaction) amplification primers, wherein the mixture of RT primers and the mixture of PCR amplification primers comprise each RT primer and each PCR amplification primer based on genetic groups, reference genes and an internal standard substance of the reaction respectively, also comprise random primers, the genetic groups comprise RRM1, TOP2A, ERCC1, TYMS, TUBB3, TOP1, PTEN, HER2, DPYD and EGFR; the reference genes comprise ACTB, GAPDH and B2M; the internal standard substance of the reaction is KAN-rRNA. The multi-gene detection kit for the selection of chemotherapy regimens, which is disclosed by the invention, can be used for simultaneously detecting the expression levels of multiple genes closely related to anti-tumor medicines systematically, and is easy to use, good in accuracy, high in detection efficiency and can be well used to guide chemotherapy medication and select chemotherapy regimens.

Description

technical field [0001] The invention relates to a gene detection kit, in particular to a kit for synchronous detection of gene expression levels related to multiple anti-tumor drugs, belonging to the technical field of medical molecular biology. Background technique [0002] As we all know, chemotherapy is the most commonly used option in cancer treatment today, but more than half of the patients often have little curative effect after chemotherapy, and the same tumor drug often has great differences in the curative effect for different patients; at the same time, the side effects of chemotherapy drugs are relatively high. Large, it kills tumor cells and normal tissue cells at the same time, and sometimes complications occur. Many patients are tortured and even die due to the strong toxic side effects of chemotherapy. A large number of clinical studies have confirmed that the treatment of tumors based on experience alone generally has a curative effect of less than 30%, and ...

AI Technical Summary

Problems solved by technology

However, if it is necessary to detect the expression of multiple genes, it is mostly necessary to detect each gene one by one, and it is difficult to set an internal reference gene, with low throughput, high cost, and poor convenience.

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