Modification method of immobilization of hydrogen-production microorganisms in sludge by agar embedding method

Abstract

The invention relates to modification of immobilization of hydrogen-production microorganisms in sludge by an agar embedding method by adding anaerobe agar and gama-alumina. The mass ratio of plain agar to anaerobe agar is 2:1-4:1; the adding amount of gama-alumina is 1.5-2.5%; the volume ratio of agar solution to sludge is 2:3-3:2; after dripping ball formation, the agar balls are inoculated in an anaerobic fermentation hydrogen production system. The anaerobe agar can provide nutrition for hydrogen-production microorganisms, shorten the adaptation period, form certain pore channels after being used by microorganisms, and thus facilitate mass transfer. The gama-alumina is nontoxic, odourless, high in hardness, large in specific surface area, strong in adsorbability, and high in solution penetration rate, facilitates microorganism adhesion, and facilitates the enhancement of the mechanical strength of agar balls and the promotion of the mass transfer process. According to the invention, when the agar embedding method is modified, the cumulative hydrogen production amount is increased, the hydrogen production time delay is shortened, the specific hydrogen production rate is increased, the hydrogen concentration in the produced gas is increased, and continuous and stable hydrogen production by the system is maintained.

Description

technical field [0001] The invention relates to a modification of a hydrogen-producing microorganism immobilization method. The adaptation time of hydrogen-producing microorganisms is shortened by adding a small amount of anaerobic bacteria agar, and the mechanical properties of agar particles are improved by adding γ-alumina. It is mainly used in anaerobic fermentation hydrogen production system to improve microbial load resistance and maintain the stability of hydrogen production system. Stablize. Background technique [0002] With the development of society and economy, people's mining and utilization of fossil fuels are increasing day by day, but the reserves of fossil fuels on the earth are limited, which leads to the "energy crisis". Moreover, the burning of fossil fuels has brought serious environmental pollution problems. Therefore, it is inevitable to seek an alternative, renewable energy source. The combustion calorific value of hydrogen is 142.35kJ / g, which is ...

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Problems solved by technology

However, the pellets obtained by this method are inconsistent in shape and size, which is not conducive to the investigation of the immobilization effect, and the loading of pure bacteria is not conducive to the diversified utilization of substrates.