Biological organic fertilizer for preventing and controlling soil successive cropping obstacles and preparation method thereof
A bio-organic fertilizer and soil continuous cropping obstacle technology, applied in the preparation of organic fertilizer, organic fertilizer, bio-organic part treatment and other directions, can solve the problems of insignificant effect, unstable effect, narrow application range, etc., and achieve remarkable effect and effect. Good, enhance the effect of disease resistance
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Embodiment 1
[0046] (1) Preparation of fermentation broth: Microbial strain ACCC10388 was subjected to liquid fermentation. The fermentation production conditions were as follows: the culture broth was made of 5 grams of peptone, 5 mg of manganese sulfate monohydrate, 1000 ml of tap water, natural pH, 121°C, 0.1MP preservation Sterilize by pressing for 30 minutes. After activating the slant of the strain, expand the culture in the shake flask for 24 hours, and then transfer it to the fermenter for culture according to the inoculation amount of 1%. The fermenter was cultured for 24 hours, the culture (fermentation) temperature was 28-30°C, the rotation speed was 200 rpm, the ventilation rate was 1:1 (volume ratio of air to fermentation broth / min); the live bacteria content of the fermentation broth was 8 ×10 9 pieces / ml.
[0047] (2) Preparation of medical stone bacteria agent: Inoculate the fermentation liquid of ACCC10388 into medical stone powder at an amount of 60L / ton, mix evenly, st...
Embodiment 2
[0053] (1) Preparation of fermentation broth: Microbial strain ACCC10388 was subjected to liquid fermentation production. The fermentation production conditions were as follows: 5 grams of peptone, 5 mg of manganese sulfate monohydrate, 1000 ml of tap water, natural pH, 121 ° C, 0.1 MP Keep the pressure for 30 minutes to sterilize. After activating the slant of the strain, expand the culture in the shake flask for 24 hours, and then transfer it to the fermenter for cultivation according to the inoculation amount of 2% (v / v); cultivate the fermenter for 24 hours, and cultivate ( Fermentation) The temperature is 28-30°C, the rotation speed is 200 rpm, the ventilation rate is 1:1 (the volume ratio of air to fermentation broth / min); the viable bacteria content of fermentation broth is 1×10 10 pieces / ml.
[0054] (2) Preparation of medical stone bacteria agent: Inoculate the fermentation liquid of ACCC10388 into medical stone powder at an amount of 50L / ton, mix evenly, stack and fe...
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