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Rapid proliferation method for anoectochilus formosanus tissue culture

A tissue culture and rapid technology, applied in horticultural methods, botanical equipment and methods, horticulture and other directions, can solve the problems of long breeding cycle, high cost, low proliferation rate of clematis, and achieve the effect of shortening the culture cycle

Active Publication Date: 2014-06-18
厦门乐莲乐生物科技有限公司
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

[0005] The object of the present invention is to provide a method for rapid proliferation of clematis tissue culture, which solves the problems of low proliferation rate, long breeding cycle and high cost of clematis, and realizes rapid industrialized breeding of clematis

Method used

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specific Embodiment

[0018] 1. Induction culture of explants

[0019] Take healthy clematis plants free from diseases and insect pests, remove the roots, root hairs, leaves and terminal buds, wash them with tap water, soak them in washing powder water for 5 minutes, then rinse them with tap water, and move them to a sterile operating table. Disinfect with 75% alcohol for 30 seconds, rinse once with sterile water, disinfect with 0.1% mercuric chloride for 3 minutes, rinse with sterile water for 3 times, disinfect with 10% sodium hypochlorite for 6-8 minutes, rinse with sterile water for 5 times, drain and put Transfer to sterile inoculation trays. Cut the sterilized stem segments into about 1 cm on the inoculation platform, and inoculate several stem segments containing one stem node in the induction medium, and each bottle receives one stem segment. If sterile seedlings are used for direct inoculation, it is only necessary to remove the leaves of the tissue cultured seedlings on the inoculation p...

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Abstract

The invention discloses a rapid proliferation method for anoectochilus formosanus tissue culture, which comprises the steps: cleaning and disinfecting an explant, inoculating the explant after cleaning and disinfecting in an MS culture medium, performing low light culture for one week, then performing strong light culture for one week, wherein the illumination time is 10 hours per day, and the temperature is 23+ / -2 DEG C; culturing for 40 days; inoculating the explants after culturing in a liquid culture medium of the MS culture medium, 1.4-1.6mg / L of 6-BA and 0.2-0.3mg / L of NAA in a ratio for enrichment culture under a dark culture condition, wherein the temperature is 23+ / -2 DEG C, and the culture period is 45 days; and by using a strong seedling culture medium as the MS culture medium, performing illumination culture, wherein the illumination time is 8 hours per day, the temperature is 23+ / -2 DEG C, the culture period is 12-15 days, and the ratio of a rooting culture medium is as follows: the content of MS is 1 / 2, the content of bananas is 60g / L, the content of active carbon is 1g / L, the content of casein hydrolysate is 0.5g / L, the illumination time is 10 hours per day, the temperature is 23+ / -2 DEG C, and the culture period is 3 months. According to the rapid proliferation method for the anoectochilus formosanus tissue culture, the industrialized rapid proliferation is realized, and the culture period is shortened.

Description

technical field [0001] The invention relates to the field of biotechnology, in particular to a rapid proliferation method for tissue culture of clematis. Background technique [0002] Anoectochilus roxburghii (Wall.) Lindl is a perennial herbaceous plant of the genus Orchidaceae. It has the functions of clearing heat and detoxifying, dispelling wind and dampness, nourishing yin and moistening lungs, and lowering blood pressure. At the same time, the clematis is small and beautiful, with beautiful leaf shape and golden veins arranged in a net shape, which has a very high ornamental value. [0003] However, the natural germination rate and reproduction rate of clematis seeds are low, and the growth is slow. In addition, a large number of human excavations and the destruction of the ecological environment have resulted in the depletion of wild clematis resources. In the prior art, asexual reproduction lines are established through tissue culture to protect and rationally utili...

Claims

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Application Information

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Patent Type & Authority Applications(China)
IPC IPC(8): A01H4/00
Inventor 石斗开陈瑞凤陈秀环赖鹭挺赖鹭捷陆利春
Owner 厦门乐莲乐生物科技有限公司
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