A closed crop leaf surface secretion secretion circulation extraction device

A cyclic extraction and secretion technology, applied in the preparation of test samples, etc., can solve the problems of reagent consumption and extraction speed, no improvement, human body harm, etc., and achieve the effect of cheap equipment, time saving and cost saving.

Active Publication Date: 2016-08-24
CHINA TOBACCO HENAN IND +1
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

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Problems solved by technology

[0003] Deficiencies in the existing extraction methods: 1) Harm to the human body
2) A large amount of reagent consumption or slow extraction speed
At present, the research work on foliage secretions is a hot topic of research by domestic and foreign scholars, but the most basic and simple extraction of foliage secretions has never been improved in domestic and foreign research since the 1990s.

Method used

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  • A closed crop leaf surface secretion secretion circulation extraction device

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Embodiment Construction

[0013] The present invention will be described in further detail below in conjunction with the accompanying drawings, but the protection scope of the present invention is not limited thereto.

[0014] Such as figure 1 As shown, a closed crop leaf surface secretions circulation extraction device includes a double-mouthed round bottom flask 1 connected in sequence from bottom to top, a separatory funnel 2 with a suction filter 13, a flat bottom funnel 3, a separator A liquid funnel 4, a tail connecting pipe 5 and a condenser pipe 6, and the branch of the double-necked round bottom flask 1 is connected to the inlet of the condenser pipe 6 through joints 7, 8 and glass tubes 9, 10. The lower end of the separating funnel 2 with the suction filter 13 is sealed and connected with the double-mouthed round-bottom flask 1 through the sleeved plug 11; the top of the flat-bottomed funnel 3 is sealed with the separating funnel 4 by setting a glass plate 12 with holes The aperture of the g...

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Abstract

The invention belongs to the technical field of extraction devices, and in particular relates to a closed-type crop leaf surface secretion circulation extraction device, which includes a double-mouthed round-bottomed flask, a separatory funnel with a suction filter, and a flat-bottomed funnel sequentially connected from bottom to top , a separatory funnel, a tail connecting pipe and a condenser tube, and the branch of the double-necked round bottom flask is connected with the outlet of the condenser tube through a joint and a glass tube. The device of the invention makes up for the harm to the body of operators and the defects of large consumption of volatile organic solvents in the existing extraction method, accelerates the extraction speed, and reduces the error of human operation. The device of the invention is cheap, easy to operate, and can safely, efficiently and quickly extract the secretions from the leaves of crops.

Description

technical field [0001] The invention belongs to the technical field of extracting devices, and in particular relates to a closed-type crop leaf surface secretion circulation extracting device. Background technique [0002] At present, the commonly used method for extracting leaf exudates is as follows: the area of ​​the leaves must be recorded before soaking. When extracting, pour about 1000ml of dichloromethane into a metal tray (clean without water residue), clamp the base of the petiole with tweezers, immerse the whole leaf in the dichloromethane solution for 2 seconds, and lift the leaf out of the solution for 2 seconds. The dichloromethane on the blade is dripped back into the dish, which is counted as one leaching, and each leaf is repeatedly leached 4 times, and the dichloromethane on the blade needs to be completely dripped back into the dish after the last leaching. Transfer all the dichloromethane in the tray to a 2L beaker, add 1ml of internal standard (if requir...

Claims

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Application Information

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Patent Type & Authority Patents(China)
IPC IPC(8): G01N1/28
Inventor 于建春王冬彭玉富杨铁钊孙九哲王根发张迪
Owner CHINA TOBACCO HENAN IND
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