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Method for constructing saury otolith physiological state analyzing model

A technology for analyzing models and physiological states, applied in fish farming, applications, climate change adaptation, etc., can solve problems such as unstandardized research and lack of unified research methods

Inactive Publication Date: 2014-06-11
SHANGHAI OCEAN UNIV
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Problems solved by technology

[0003] The purpose of the present invention is to provide a method for constructing an analysis model of the physiological state of saury otoliths, aiming to solve the problems of lack of unified research methods and unstandardized research

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  • Method for constructing saury otolith physiological state analyzing model

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Embodiment 1

[0026] A method for constructing a physiological state analysis model of saury otoliths, comprising the construction of a saury otolith growth analysis model, the construction method of the saury otolith growth analysis model comprising the following steps:

[0027] (1) Extract the biological data of saury, and extract the otoliths from the balance sac of the head of saury, number and save them;

[0028] In step (1), the biological data of saury include body length, weight, fork length, sex, and gonad maturity of saury; the preservation is to preserve otoliths in 75% ethanol solution.

[0029] (2) Use the microscopic imaging system CCD to take pictures of the otoliths, and measure the morphological parameters of the saury otoliths in combination with the pictures taken under microscope observation;

[0030] The morphological parameters include: L1: the long axis of the otolith (TSL), L2: the short axis of the otolith (TSH), L3: the vertical distance from the center of the otol...

Embodiment 2

[0045] In the further implementation process, in order to analyze the physiological state of saury more comprehensively, in the embodiment of the present invention, the method for constructing the physiological state analysis model of saury otoliths also includes the age analysis of the otolith ring pattern of saury The construction of model, the construction method of described Pacific saury otolith ring pattern age analysis model comprises the following steps:

[0046] (4) Embedding, roughly grinding and polishing the otoliths in step (1);

[0047] In step (4), the embedding is to embed the otoliths in the step (1) with polyester resin in the embedding box and solidify; the rough grinding is to use No. 600 sandpaper under microscope observation Grinding the surface of the otolith, the grinding depth does not exceed the core part of the otolith; the polishing is under microscope observation, using No. 1000 sandpaper for primary polishing, and then using a polishing flannelett...

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Abstract

The invention provides a method for constructing a saury otolith physiological state analyzing model. The method comprises the steps of extracting biological data of saury, numbering and storing otolith after extracting the otolith from a balance bag part in the head of the saury, photographing the otolith by utilizing a micro imaging system CCD, and measuring various form parameters of saury otolith by combining a microscope observation and photographing picture; carrying out main component form characteristic index clustering analysis on the form parameters by utilizing a gray constellation clustering method, obtaining main component form parameters, and obtaining a change mode of otolith growth speed; carrying out embedding, rough grinding and polishing treatment on the otolith; interpreting and counting annular ring grains of the polished otolith, photographing by using the CCD, and judging age data of the otolith according to an interpreting and counting result; analyzing the age data and the obtained biological data, and obtaining a growth equation. According to the method provided by the invention, the saury otolith physiological state can be accurately analyzed, and the method has the effect of scientific research standard.

Description

technical field [0001] The invention belongs to the technical field of biological development model construction, and in particular relates to a method for constructing a physiological state analysis model of saury otoliths. Background technique [0002] The otoliths of saury can be used for its age identification, and its morphological research can lay the foundation for this. However, there are few domestic studies on it, and there is no unified research method. Contents of the invention [0003] The purpose of the present invention is to provide a method for constructing a physiological state analysis model of saury otoliths, aiming to solve the problems of lack of unified research methods and unstandardized research. [0004] The present invention is realized in this way, a kind of method of constructing saury otolith physiological state analysis model, comprises the construction of saury otolith growth condition analysis model, the construction method of described sau...

Claims

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Application Information

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IPC IPC(8): A01K61/00
CPCY02A40/81
Inventor 朱清澄王晓杰张阳花传祥高玉珍
Owner SHANGHAI OCEAN UNIV
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