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Method for promoting hardening-off and rooting of dendrobium tissue culturing seedlings

A technology for strong seedling rooting and tissue culture seedlings, which is applied in the field of plant biology, can solve the problems of large differences in the purity and effective concentration of extracts, inability to store for a long time, poor root growth, etc., shorten the time for rooting induction, and the operation method is simple and easy line, low cost effect

Inactive Publication Date: 2014-06-11
SHANGHAI ACAD OF AGRI SCI
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

We added banana juice and potato juice to the tissue culture of orchid plants such as Cymbidium cymbidium, Qiandailan, Dendrobium officinale, and Dendrobium spp. plant growth
[0008] In addition, due to the different extraction methods of natural extracts, the maturity and physiological state of the extracted materials, the purity and effective concentration of the extracts vary greatly, which will have an impact on the experimental results
Natural extracts need to be extracted immediately after each use, and cannot be stored for a long time, and the operation is more troublesome

Method used

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  • Method for promoting hardening-off and rooting of dendrobium tissue culturing seedlings
  • Method for promoting hardening-off and rooting of dendrobium tissue culturing seedlings
  • Method for promoting hardening-off and rooting of dendrobium tissue culturing seedlings

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Experimental program
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Effect test

Embodiment 1

[0046] Dendrobium officinale tissue culture seedling strong seedling rooting culture method of the present embodiment, concrete steps are as follows:

[0047] 1) Take the 2cm high Dendrobium officinale tissue culture seedlings (without roots) from the stem explants, cut the seedlings under aseptic conditions, transfer them to culture bottles equipped with strong seedling rooting medium, and place the culture bottles in light Cultivate under conditions, the culture temperature is 25°C±2°C, the light time is 16 hours / day, and the light intensity is 1000lux; the formula of rooting and strong seedling medium is MS basic medium+5mg / LHMI+5g / L agar powder+30g / L sucrose.

[0048] After 4-5 days of cultivation, roots will appear, and after one month of cultivation, the statistical rooting rate will reach 100% (see figure 1 ), with an average of 6-8 roots per plant, and an average root length and plant height of 7.1-7.8cm (see figure 2 ), the leaf color is dark green, the leaves are...

Embodiment 2

[0051] Dendrobium officinale tissue culture seedling strong seedling rooting culture method of the present embodiment, concrete steps are as follows:

[0052] 1) Take the 2cm high Dendrobium officinale tissue culture seedlings (without roots) from the stem explants, cut the seedlings under aseptic conditions, transfer them to culture bottles equipped with strong seedling rooting medium, and place the culture bottles in light Cultivate under conditions, the culture temperature is 25°C±2°C, the light time is 16 hours / day, and the light intensity is 1000lux; the formula of rooting and strong seedling medium is MS basic medium + Huoshang Aoyou 12-element liquid compound fertilizer 1000 times +5g / L agar powder+30g / L sucrose;

[0053] After 15 days of cultivation, there were roots, and after 45 days of cultivation, the statistical rooting rate reached 100%, with an average of 7-8 roots per plant, and the roots were strong and neat (see Figure 4 ), the average plant height is 8.1-8...

Embodiment 3

[0056] Dendrobium officinale tissue culture seedling strong seedling rooting culture method of the present embodiment, concrete steps are as follows:

[0057] 1) Take the 2cm high Dendrobium officinale tissue culture seedlings (without roots) from the stem explants, cut the seedlings under aseptic conditions, transfer them to culture bottles equipped with strong seedling rooting medium, and place the culture bottles in light Cultivate under conditions, the culture temperature is 25°C±2°C, the light time is 16 hours / day, and the light intensity is 1000lux; the formula of rooting and strong seedling medium is MS basic medium + 5mg / LHMI + Huoshang Aoyou 12-element liquid compound Fertilizer 1000 times + 5g / L agar powder + 30g / L sucrose;

[0058] Cultivate 5 days to have the generation of root, cultivate after 25 days and count the rooting rate to reach 100%, averagely every plant takes root 7-8, and root is thick and strong, orderly, and plant growth vigor is stronger than only a...

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Abstract

The method discloses a method for promoting hardening-off and rooting of dendrobium tissue culturing seedlings. The method comprises the following steps: 1, transferring the dendrobium tissue culturing seedlings to a specific hardening-off and rooting culture medium for illumination culture, and performing hardening-off and root induction; 2, hardening the rooted tissue culturing seedlings and then transplanting; and 3, transplanting regenerated plants of the rooted dendrobium tissue culturing seedlings to a culture medium mixing bark with vermiculite. The method is simple to operate and is feasible and quick in hardening-off, each plant is 3-4 cm higher than that of a control group, the diameter of the stem is 2-3mm larger than that of the control group, the fresh weight is 3 times larger than that of the control group, early rooting can be realized, roots can be formed in 4-5 days, the rooting rate is as high as 100% within 30 days, each plant can take 7-8 roots, and the transplanting survival rate is as high as 95%; the time for root induction can be effectively shortened, the root development is good, the plants are robust, the transplanting survival rate is high, and important reference value is achieved for culture of other orchidaceae tissue culturing seedlings.

Description

technical field [0001] The invention belongs to the field of plant biotechnology, in particular relates to tissue culture of dendrobium orchids, in particular to a method for promoting the growth and rooting of tissue cultured seedlings of dendrobium orchids. Background technique [0002] Dendrobium orchids (D.orchids) are perennial herbaceous plants of the genus Dendrobium in the family Orchidaceae, and their main uses are ornamental and medicinal. Dendrobium orchid is one of the four most famous orchids in the world and has the most ornamental value. It has many excellent characteristics such as various types, gorgeous colors, graceful posture, many and dense flowers, and long flowering period. It can be used as potted plants and cut flowers. It is suitable for large Intensive commodity production in some areas has broad application prospects. Dendrobium orchid is also a traditional precious Chinese medicinal material in my country, such as the famous Dendrobium officinal...

Claims

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Application Information

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IPC IPC(8): A01H4/00
Inventor 周音张建军陈敏敏谢纪红
Owner SHANGHAI ACAD OF AGRI SCI
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