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Rooting culture method of angelica sinensis tissue culture seedlings

A technology of rooting culture and rooting medium, which is applied in the field of agricultural biology, can solve the problems of not strong adventitious roots, low rooting rate of angelica tissue culture seedlings, and low transplanting survival rate, so as to achieve a well-developed root system, promote the growth of adventitious roots, and increase the survival rate Effect

Inactive Publication Date: 2020-08-14
INST OF ECONOMIC CROPS & BEER RAW MATERIAL GANSU ACADEMY OF AGRI SCI
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

[0006] The present invention aims to solve the deficiencies in the above-mentioned process, overcome the problems of low rooting rate of angelica tissue culture seedlings under the existing conditions, weak adventitious roots, and low transplanting survival rate, and provides a plant with high rooting rate, well-developed root system and transplanting survival rate. Rooting culture method of Angelica sinensis tissue culture seedling

Method used

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  • Rooting culture method of angelica sinensis tissue culture seedlings
  • Rooting culture method of angelica sinensis tissue culture seedlings
  • Rooting culture method of angelica sinensis tissue culture seedlings

Examples

Experimental program
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Effect test

Embodiment 1

[0042] (1) Select robust 1cm~3cm high self-rooted seedlings, cut it into single buds under aseptic conditions, inoculate to rooting medium (1 / 2MS (solid medium)+1.0mg / LIBA), and The light is 12h / d, the light intensity is 2000lx, the culture temperature is 23±1°C, and the rooting is induced in an environment with a relative humidity of 50-60%. The test-tube seedling plant seedling growth after rooting culture 6 weeks is robust, and root system is developed, and rooting rate reaches 100%, see figure 1 ;

[0043](2) seedling hardening: the opening time of the first day's culture bottle cap is 2 hours, 1 hour in the morning and 1 hour in the afternoon; the opening time of the second day's culture bottle cap is 4 hours, 2 hours in the morning and 2 hours in the afternoon; , 3 hours in the morning, 3 hours in the afternoon, and 2 hours in the evening; on the fourth day, the opening time of the culture bottle cap was 12 hours, and the opening interval was 4 hours every 4 hours; on t...

Embodiment 2

[0046] (1) Select robust 1cm-3cm high self-rooted seedlings, cut them into single buds under aseptic conditions, and inoculate them into rooting medium (MS (solid medium)+1.0mg / LIBA+1.5mg / LNAA ), rooting is induced in an environment where the light is 12h / d, the light intensity is 1500lx, the culture temperature is 23±1°C, and the relative humidity is 50-60%. After rooting and culturing for 4 weeks, the test-tube seedlings grow robustly, with well-developed root system and a rooting rate of 90%;

[0047] (2) seedling hardening: the opening time of the first day's culture bottle cap is 2 hours, 1 hour in the morning and 1 hour in the afternoon; the opening time of the second day's culture bottle cap is 4 hours, 2 hours in the morning and 2 hours in the afternoon; , 3 hours in the morning, 3 hours in the afternoon, and 2 hours in the evening; on the fourth day, the opening time of the culture bottle cap was 12 hours, and the opening interval was 4 hours every 4 hours; on the fif...

Embodiment 3

[0050] (1) Select robust 1cm~3cm high self-rooted seedlings, cut them into single buds under aseptic conditions, inoculate them into rooting medium (MS (solid medium)+1.0mg / LIBA), and place them under light for 12h / d, the light intensity is 2000lx, the cultivation temperature is 23±1°C, and the relative humidity is 50-60% to induce rooting. After rooting and culturing for 5 weeks, the test-tube seedlings grow robustly, with well-developed root system and a rooting rate of 85%;

[0051] (2) seedling hardening: the opening time of the first day's culture bottle cap is 2 hours, 1 hour in the morning and 1 hour in the afternoon; the opening time of the second day's culture bottle cap is 4 hours, 2 hours in the morning and 2 hours in the afternoon; , 3 hours in the morning, 3 hours in the afternoon, and 2 hours in the evening; on the fourth day, the opening time of the culture bottle cap was 12 hours, and the opening interval was 4 hours every 4 hours; on the fifth day, the opening...

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Abstract

The invention provides a rooting culture method of angelica sinensis tissue culture seedlings. The method comprises the following steps of cutting 1-3cm high slave rootless seedlings into single buds,transferring the single buds into a rooting culture medium, and inducing rooting under the illumination condition to obtain complete test-tube plantlet plants, and carrying out acclimatization and transplantation to perform field transplantation. According to the rooting culture method for the angelica sinensis tissue culture seedlings, the rooting rate can reach 85% or above, the transplanting survival rate is 75% or above, the rooting induction time can be effectively shortened, the adventitious root growth can be promoted, induced roots and root systems are developed, and the seedlings arerobust.

Description

technical field [0001] The invention belongs to the field of agricultural biotechnology, and in particular relates to the tissue culture of Chinese medicinal material Angelica sinensis, in particular to a rooting culture method for tissue cultured seedlings of Angelica sinensis. Background technique [0002] Angelica sinensis (Oliv) Diels is a perennial herb of Umbelliferae, and its dry root is a commonly used Chinese medicinal material in my country. Angelica has the effects of invigorating blood circulation, regulating menstruation, relieving pain, moistening intestines and laxative, and is known as "ten directions and nine returns". Angelica is not only used for curing diseases, but also for health care and beauty. It is also one of the main medicinal plants for foreign exchange earning in my country, and its demand is increasing year by year. [0003] The growth cycle of Angelica sinensis in artificial cultivation is 3 years. Conventional methods of breeding or raising ...

Claims

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Application Information

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Patent Type & Authority Applications(China)
IPC IPC(8): A01H4/00
CPCA01H4/008A01G7/02A01G31/00A01G22/25Y02P60/21
Inventor 蔡子平王宏霞王国祥
Owner INST OF ECONOMIC CROPS & BEER RAW MATERIAL GANSU ACADEMY OF AGRI SCI
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