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Method for extraction of white blood cells from blood clot

A blood clot and white blood cell technology, applied in the field of white blood cell extraction, can solve the problems of white blood cell extraction, and achieve the effect of meeting scientific research and clinical needs, sufficient yield, and complete surface molecular structure

Inactive Publication Date: 2014-05-28
SHANXI MEDICAL UNIV
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

[0006] The purpose of the present invention is to solve the existing technical problem that white blood cells cannot be extracted from blood clots, and provide a method for extracting white blood cells from blood clots

Method used

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  • Method for extraction of white blood cells from blood clot
  • Method for extraction of white blood cells from blood clot

Examples

Experimental program
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Embodiment 1

[0028] The method for extracting white blood cells from a blood clot in this embodiment includes the following steps:

[0029] 1) According to the size of the clot remaining after the serum is separated, select a beaker or a plate with a suitable caliber, cover the upper mouth of the selected container with a 100-mesh filter of a suitable size, and arrange the filter to make it tightly integrated with the container mouth;

[0030] 2) Peel off and transfer the clot to the filter prepared above;

[0031] 3) Use a 5ml glass syringe core or a smooth grinding stick to gently grind the clot on the filter screen, and use a straw or dropper to continuously add saline to moisten and rinse the clot and the grinding tools that come into contact with the clot. The blood cells are released from the fibrous network of the clot, and pass through the filter to collect in the container. The saline washes the grinding tool and the filter several times until only the filamentous coagulation fiber is le...

Embodiment 2

[0035] The method for extracting peripheral blood mononuclear cells from a blood clot in this embodiment includes the following steps:

[0036] The blood cells collected in step 1) to step 4) in Example 1 were diluted with normal saline to two times the original blood volume, and the peripheral blood mononuclear cells were separated with conventional lymphocyte layering solution.

[0037] If the leukocytes or peripheral blood mononuclear cells obtained in the above embodiments are required to be sterile, they need to be autoclaved in advance to process the container with the embedded filter and other experimental equipment used in the method, and the operation steps also need to be sterilized.

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Abstract

The invention provides a method for extraction of white blood cells from a blood clot, comprising the following steps: covering a 100 mesh filter screen onto a beaker or plate; stripping the blood clot and transferring it onto the filter screen; grinding the blood clot in the core of a glass syringe and dropping normal saline to wash simultaneously; gathering blood cells which pass through the filter screen and collect in the beaker or plate; adding hemolysin to crack red blood cells in blood cells and obtaining white blood cells; or separating blood cells by lymphocyte stratified fluid to obtain peripheral blood mononuclear cells. The invention is applicable to the field of scientific experiments or clinical tests in which both serum and white blood cells are needed according to the same blood sample.

Description

Technical field [0001] The invention relates to a method for extracting white blood cells from blood clots. Background technique [0002] Scientific experiments and clinical tests often encounter situations where both serum and white blood cells are needed for the same blood sample for related experiments or tests. However, after the serum is separated from the coagulated blood, the white blood cells imprisoned in the clot can no longer be used. Therefore, when both serum and white blood cells are needed, clinical and scientific research often need to increase the blood collection volume and divide the blood obtained into two. One part of coagulated blood is used to separate serum, and the other part of anticoagulated blood is used to extract white blood cells, nucleic acids or cellular proteins. [0003] In animal-related immunology scientific research, the amount of blood obtained from immunized animals often cannot meet the needs of scientific research. Especially when the blo...

Claims

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Application Information

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Patent Type & Authority Applications(China)
IPC IPC(8): C12N5/078
Inventor 樊卫平覃艳红李静
Owner SHANXI MEDICAL UNIV
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