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High-temperature cellulose degradation bacterium and application thereof

A cellulose-degrading bacteria and cellulose-degrading technology, applied in the field of environmental microorganisms, can solve problems such as decreased thermal stability, and achieve the effects of low cost, fast growth and good performance

Active Publication Date: 2014-05-28
SHENZHEN TECHAND ECOLOGY & ENVIRONMENT CO LTD
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

Xue Qiaoli et al. (2012) isolated 20 strains of high-temperature-resistant cellulolytic bacteria from compost, and the cellulolytic enzymes produced by them had good thermal stability at 50°C, but as the temperature increased, the thermal stability showed a downward trend.

Method used

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  • High-temperature cellulose degradation bacterium and application thereof
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  • High-temperature cellulose degradation bacterium and application thereof

Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0021] Example 1: Screening and identification of high temperature resistant cellulose degrading bacteria

[0022] Weigh 10 g of garden waste compost samples in the high-temperature period, place them in a conical flask filled with 10 glass beads and 90 ml of sterile water, and shake them on a shaker at 200 rpm for 30 min to fully disperse the samples. At ℃, static enrichment culture was carried out for 12 hours. Use a sterile pipette to draw 1 ml of the supernatant and transfer it to the Hutchinson culture medium, and culture at 70°C for about 30 days. Take 10 ml of the acclimatization solution and transfer it to a new Hutchinson culture solution, and continue the acclimatization culture, so that it can be passaged for 3 times.

[0023] The ingredients of the Hutchinson medium above are: Potassium dihydrogen phosphate 1.0g, Sodium chloride 0.1g, Sodium nitrate 2.5g, Magnesium sulfate 0.3g, Ferric chloride 0.01g, Calcium chloride 0.1g, Starch-free filter paper 10g, 1000ml ...

Embodiment 2

[0040] Embodiment 2: the preparation of solid bacterial agent

[0041] (1) slant activation: take the slant of the microorganisms of the present invention stored at 4°C and inoculate them into LB solid slant medium, and culture them in a thermostat at 70°C for 18 hours to realize the activation of the slant.

[0042] (2) Preparation of seed solution: Inoculate one slant of the strain activated by the slant in step (1) into 1 L of sterile LB liquid culture medium, and culture it on a water bath shaker with a culture temperature of 70°C and an oscillation frequency of 100 rpm, the seed solution was obtained after culturing for 30 h.

[0043] (3) Preparation of fermented seed liquid: the above seed liquid was inoculated into a sterilized fermenter at an inoculum amount of 10% (v / v) for expanded fermentation culture. Under the conditions of temperature 70°C and shaking frequency 120 rpm, fermented seed liquid was obtained after culturing for 48 hours.

[0044] (4) Preparation ...

Embodiment 3

[0045] Embodiment 3: Effect test applied to high temperature composting

[0046] Use garden waste as the main composting material, supplemented with fresh chicken manure, so that the carbon-nitrogen ratio of the mixed material is 25-35:1, and the moisture content is 55-60%. When the maximum temperature of the compost rises to 55°C, the solid bacterial agent prepared in Example 2 is inoculated into the compost material according to the proportion of 15% of the total material, and the carbon-nitrogen ratio is adjusted to 35:1, and the moisture content is 55%. 50d of compost. Simultaneously, the test was carried out without adding the microbial inoculant of the present invention as a control.

[0047] Heap temperature detection: measure once a day for the first 15 days, and measure once every 5 days after that. Turn the pile once every 5 days, turn the pile when the pile temperature reaches 70°C, and keep the pile body temperature between 50-70°C during the high temperature s...

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Abstract

The invention discloses a high-temperature cellulose degradation bacterium and the application thereof. The high-temperature resistant cellulose degradation bacterium provided by the invention can grow within a temperature range of 60-80 DEG C and grows fastest at 70 DEG C. The cellulose degradation bacterium which has a stable cellulose degradation function can faster adapt to high-temperature compost environment, can fast degrade organic compounds which are difficult to degrade, such as cellulose and the like, and can accelerate the process that compost becomes thoroughly decomposed. Solid-state microbial inoculum which is prepared by bacterial strains is used to compost garden waste, and compared with nonvaccinated cellulose, the total degradation rate is increased by 42.2%. The cellulose degradation bacterium has the advantages of low cost, good performance and the like.

Description

Technical field [0001] The present invention is an environmental microorganism, which involves a cellulose degradation bacteria that can grow rapidly at 70 ° C and its application in high temperature compost of garden waste. Background technique [0002] Leaf composting is one of the common technologies to achieve harmlessness, reduction and resourceization of organic waste.At present, the problem of long fermentation cycle, incomplete organic degradation of organic substances, low material stability and low corruption.To this end, people will separate high -temperature bacteria, medium temperature bacteria, threading bacteria, and fungal fungi from the environment and apply them in the compost system to achieve the purpose of shortening and fermentation time and increasing the cookedness of composting.Garden waste includes dead branches, lawn pruning, weeds, and residual flowers. It is a common compost material and cellulose is its main component.Due to the dense structure of th...

Claims

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Application Information

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IPC IPC(8): C12N1/20C05F11/08C05F17/00C12R1/01
CPCY02W30/40
Inventor 于光辉翟晓峰张英严建平余震喻薇田军建高强
Owner SHENZHEN TECHAND ECOLOGY & ENVIRONMENT CO LTD
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