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Method for making large helminth specimen

A parasite and specimen technology, applied in the field of medical human parasitology, can solve the problems of poisoning, difficult to observe, volatile and other problems of the preservation solution, and achieve the effects of good use effect and efficiency, diverse specimen shapes and strong transparency.

Inactive Publication Date: 2014-05-28
QINGDAO UNIV
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

The specimens preserved by this method are easily deformed and discolored; the preservation solution is toxic and volatile; the specimens are bulky and difficult to observe, and many other disadvantages

Method used

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Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0011] Gross specimen preparation of Hymenoleta adult worms, the steps are as follows:

[0012] a. Selection and collection of adults: select a complete worm body with a length of about 5 cm and active movement, and wash it with warm saline at 37°C;

[0013] b. Anesthesia and pre-cooling: Put the worms in a 25% alcohol solution for 5 minutes, take out the worms after anesthesia, and put them in a -20°C refrigerator for 30 minutes to pre-cool;

[0014] c. Freeze drying: put the worm body in a vacuum freeze dryer for 20 hours;

[0015] d. Epoxy resin embedding: Put the insect body into a cuboid mold of 8cm×3cm×3cm, mix the two-component epoxy resin A and B components in proportion, slowly pour it into the mold, and wait for it to completely cure.

Embodiment 2

[0017] To prepare the gross specimen of Fasciola brucei adults, the steps are as follows:

[0018] a. Selection and collection of adults: select a complete worm body with a length of about 6 cm and active movement, and wash it with warm saline at 37°C;

[0019] b. Anesthesia and pre-cooling: Put the worms in a 30% alcohol solution for 7 minutes, take out the worms after anesthesia, and put them in a -20°C refrigerator for 30 minutes to pre-cool;

[0020] c. Freeze-drying: put the worm body in a vacuum freeze dryer for 24 hours;

[0021] d. Epoxy resin embedding: Put the worm body into a cuboid mold of 10cm×5cm×3cm, mix the two-component epoxy resin A and B components in proportion, slowly pour into the mold, and wait for it to completely cure.

Embodiment 3

[0023] Gross specimen preparation of worm Ascaris larvae, the steps are as follows:

[0024] a. Selection and collection of adults: select a complete worm body with a length of about 10 cm and active movement, and wash it with warm saline at 37°C;

[0025] b. Anesthesia and pre-cooling: Put the worms in a 30% alcohol solution for 10 minutes, take out the worms after anesthesia, and put them in a -20°C refrigerator for 30 minutes to pre-cool;

[0026] c. Freeze drying: put the worm body in a vacuum freeze dryer for 30 hours;

[0027] d. Epoxy resin embedding: Put the worm body into a cuboid mold of 15cm×3cm×3cm, mix the two-component epoxy resin A and B components in proportion, slowly pour into the mold, and wait for it to completely cure.

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PUM

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Abstract

The invention belongs to the field of medical human parasitology and provides a method for making a large helminth specimen. The method comprises the following steps: specimen selection and collection; anesthetizing and precooling; freeze drying; epoxy resin embedding. The large helminth specimen made by using the method is capable of keeping the integrity of the appearance and shape of a helminth body for a long term, convenient to carry, diversified in appearance, free of preservative and pollutant volatilization, strong in transparency and easy to observe so as to meet the application requirement of parasitology in teaching and research processes and obtain better using effect and efficiency.

Description

technical field [0001] The invention belongs to the field of medical human parasitology, and relates to a method for making gross specimens of worm parasite larvae and adults, which can be used for making gross specimens of parasites, especially in teaching and scientific research of human parasitology. Background technique [0002] At present, the production of gross specimens of parasites at home and abroad still uses the traditional production method. After the parasites are processed, they are soaked in 5% to 10% formalin solution and stored in transparent glass or plexiglass bottles. The specimens preserved by this method are easily deformed and discolored; the preservation solution is poisonous and volatile; Contents of the invention [0003] Aiming at the deficiencies in the prior art, the object of the present invention is to provide a method for making a general parasite specimen that can preserve parasites for a long time, is portable, and is easy to observe. ...

Claims

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Application Information

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Patent Type & Authority Applications(China)
IPC IPC(8): A01N1/00G09B23/36
Inventor 常志尚张忠广赵蓉曾宪忠申成华
Owner QINGDAO UNIV
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