Detection method and kit of HLA (Human Leukocyte Antigen)-B*58:01 allele

Abstract

The invention relates to a detection method of HLA (Human Leukocyte Antigen)-B*58:01 allele. The detection method comprises the following steps: providing a mixture of a sample to be tested, a nucleic acid amplification system and a fluorescence detection system; circularly amplifying target polynucleotide through an amplified reaction; indirectly combining a fluorescence generating group and an amplified target polynucleotide sequence; and detecting the fluorescent amount generated by the fluorescence generating group so as to determine existence of the target polynucleotide and the relative amount thereof. The invention further discloses a kit for detecting the allele. The kit comprises a plurality of sealed centrifuge tubes which are respectively filled with a reaction liquid 1, a reaction liquid 2, a reaction liquid 3, an EZ Taq enzyme mixed liquid, a standard liquid 1 and a standard liquid 2 as well as the kit which separates and packages the centrifuge tubes in a centralized manner. The kit and the detection method provided by the invention are simple and convenient to operate, short in time consumed, strong in specificity and high in sensitivity. The kit and the detection device can be widely applied to detecting the HLA-B*58:01 allele and clinically avoiding severe untoward effects of skins of the HLA-B*58:01 allele patients caused by using allopurinol.

Description

Technical field [0001] The present invention relates to a method for detecting HLA-B*58:01 alleles, and a kit used for the method, in particular to the use of real-time quantitative fluorescent polymerase chain reaction technology to detect HLA-B*58:01 alleles And apply the test results to clinical methods and kits to guide the use of allopurine drugs. Background technique [0002] Adverse drug reactions (adverse drug reactions, referred to as ADR), in accordance with the provisions of the WHO International Drug Surveillance Cooperative Center, refer to normal doses of drugs used to prevent, diagnose, treat diseases or regulate physiological functions that are harmful and have nothing to do with the purpose of medication Reaction. Adverse drug reactions are huge hidden dangers in the treatment of patients. According to statistics, the total number of people who die from irrational drug use in the world reaches 19 million every year. 2.2 million people in the U.S. experience si...

AI Technical Summary

Problems solved by technology

However, while having many advantages, this method also has disadvantages such as cumbersome operation, long time consumption and high cost.

In particular, the shortcomings of cumbersome operation and long time consumption have gradually failed to meet the needs of modern medical testing, and do not adapt to the "fast and simple" concept required by modern medical testing.

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